Qin et al. isolated Alstoniascholarines L–Q (43–48, Fig. 14.7) from the
methanolic extract of A. scholaris inadequately dried leaves (Qin et al. 2015b). The
isolated new alkaloids were screened for cytotoxicity assay in various cell lines
(human colon adenocarcinoma (SW-480), human hepatocarcinoma (SMMC-7721),
HL-60, MCF-7, and human lung epithelial (A-549), by using cisplatin and paclitaxel as a positive control. Further, neurotrophic activities were evaluated in PC12
cells. Unfortunately, the alkaloids neither showed cytotoxic activity nor exhibited
neurite outgrowth-promoting activity.
Echitamine Chloride (42)
N
H
N
OH
COOCH 3
HO
Cl
Fig. 14.6 Chemical structure
of echitamine chloride (42)
Table 14.4 Cytotoxicity
effects of echitamine chloride
(42) against various cell lines
Cell lines
IC 50 of 42 (µg/mL)
KB
10.5
HeLa
30
HepG 2
50
HL-60
51
MCF-7
52
HN
O
HN
O
O
OH
HN
O
HN
O
O
OH
HN
O
N
O
O
OH
N
H
N
H
OH
COOMe
H
HO
N
H
N
H
OH
COOMe
H
HO
N
H
N
H
OH
COOMe
H
MeO
O
O
Alstoniascholarine L (43)
Alstoniascholarine M (44)
Alstoniascholarine N (45)
Alstoniascholarine O (46)
Alstoniascholarine P (47)
Alstoniascholarine Q (48)
Fig. 14.7 Chemical structures of various alkaloids from A. scholaris (43–48)
14 Pharmaceutical Application of Bio-actives …
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