activity were checked and evaluated for/by elicited explant. The outcomes revealed
that for shade grown plant, somatic embryos and light induced and dark induced
conditions antioxidative efficiencies against DPPH and ferric reducing antioxidative
processes were observed with greater efficiency in MJ, salicylic acid and glutathione elicitors. Maximum effects as antioxidative and enzymatic activities
against glutathione peroxidase, glutathione reductase and glutathione-S-transferase
were observed with salicylic acid as elicitor. These data correlated abiotic elicitor
and secondary metabolites accumulation in Trifolium resupinatum plant (Twaij
et al. 2019).
1.4.1.15 Effect of MJ Abiotic Elicitor on Anthraquinone Production
in Rubia tinctorum
Perassolo et al. experimentally proved the effects of combined culture medium and
MJ as abiotic elicitor on the production of anthraquinone in Rubia tinctorum plant.
The process was started with culturing of young small clones of leaf margin or
aerial stem of the plant with Agrobaterium rhizogenes in Gamborg B5 medium with
sucrose and ampicillin as source of carbon and strengthening of natural defense
system. Then after two to four weeks roots were developed for cloning in Gamborg
B5 and Llyod-McCown woody plant medium with thiamine, pyridoxine, nicotinic
acid and myoinositol as growth regulators. After fourteen days, inoculum cultures
were elicited in presence of MJ (100 µM) concentration. After a time interval of 0,
2, 4 and 7 days, the amount of anthraquinone and growth of biomass were evaluated. The outcomes revealed that after six weeks of treatment biomass in woody
plant medium was higher than Gamborg medium and for anthraquinone production
Gamborg medium was perfectly suited. But after seven days treatment with MJ,
anthraquinone content was remarkably good as compare to dimethylsulfoxide.
These information stated the importance of MJ and growth regulators for the
production of anthraquinone (Perassolo et al. 2017).
1.4.1.16 Effect of Polyunsaturated Fatty Acids on Gymnemic Acid
Production in Gymnema Sylvestre
Praveen et al. scientifically proved the importance of polyunsaturated fatty acids
(oleic acid and linoleic acid) on the production of gymnemic acid present in
Gymnema sylvestre plant. The experiment was started with formation of hairy root
culture of the plant with the plantation in MS medium with sucrose as carbon
source and these were sub-cultured for fortnight period. After the incubation period,
cultures were elicited with oleic acid and linoleic acid with concentration range (0,
1, 5, 10 and 50) µM. The dry biomass, amount of gymnemic acid, total phenolic
content, total flavonoid content were checked as well as antioxidative property was
also evaluated. The outcomes revealed that the growth ratio between fresh and dry
biomass was higher with linoleic acid (1.0 µM) elicitation. Highest amount of
14
S. Saha and D. Pal
that for shade grown plant, somatic embryos and light induced and dark induced
conditions antioxidative efficiencies against DPPH and ferric reducing antioxidative
processes were observed with greater efficiency in MJ, salicylic acid and glutathione elicitors. Maximum effects as antioxidative and enzymatic activities
against glutathione peroxidase, glutathione reductase and glutathione-S-transferase
were observed with salicylic acid as elicitor. These data correlated abiotic elicitor
and secondary metabolites accumulation in Trifolium resupinatum plant (Twaij
et al. 2019).
1.4.1.15 Effect of MJ Abiotic Elicitor on Anthraquinone Production
in Rubia tinctorum
Perassolo et al. experimentally proved the effects of combined culture medium and
MJ as abiotic elicitor on the production of anthraquinone in Rubia tinctorum plant.
The process was started with culturing of young small clones of leaf margin or
aerial stem of the plant with Agrobaterium rhizogenes in Gamborg B5 medium with
sucrose and ampicillin as source of carbon and strengthening of natural defense
system. Then after two to four weeks roots were developed for cloning in Gamborg
B5 and Llyod-McCown woody plant medium with thiamine, pyridoxine, nicotinic
acid and myoinositol as growth regulators. After fourteen days, inoculum cultures
were elicited in presence of MJ (100 µM) concentration. After a time interval of 0,
2, 4 and 7 days, the amount of anthraquinone and growth of biomass were evaluated. The outcomes revealed that after six weeks of treatment biomass in woody
plant medium was higher than Gamborg medium and for anthraquinone production
Gamborg medium was perfectly suited. But after seven days treatment with MJ,
anthraquinone content was remarkably good as compare to dimethylsulfoxide.
These information stated the importance of MJ and growth regulators for the
production of anthraquinone (Perassolo et al. 2017).
1.4.1.16 Effect of Polyunsaturated Fatty Acids on Gymnemic Acid
Production in Gymnema Sylvestre
Praveen et al. scientifically proved the importance of polyunsaturated fatty acids
(oleic acid and linoleic acid) on the production of gymnemic acid present in
Gymnema sylvestre plant. The experiment was started with formation of hairy root
culture of the plant with the plantation in MS medium with sucrose as carbon
source and these were sub-cultured for fortnight period. After the incubation period,
cultures were elicited with oleic acid and linoleic acid with concentration range (0,
1, 5, 10 and 50) µM. The dry biomass, amount of gymnemic acid, total phenolic
content, total flavonoid content were checked as well as antioxidative property was
also evaluated. The outcomes revealed that the growth ratio between fresh and dry
biomass was higher with linoleic acid (1.0 µM) elicitation. Highest amount of
14
S. Saha and D. Pal