was utilized as source of carbon and etephon was a plant growth regulator. The
outcomes revealed that after 5th day of treatment maximum fresh and dry weight of
the callus was observed with activated charcoal and etephon treatments. Also the
amount of anthocyanins were increased with etephon and activated charcoal
treatments. But the amount of extracellular 3-O-glucosyl resveratrol and phenolic
compound were increased with the combination treatment of etephon and high
pressure. These data confirmed the importance of abiotic elicitors on the growth and
accumulation of secondary metabolites present in Vitis vinifera plant (Cai et al.
2011).
1.4.1.10 Effect of Salicylic Acid on Alkaloid Biosynthesis in Marine
Microalgae Arthrospira platensis
Hadizadeh et al. experimentally proved the importance of salicylic acid as abiotic
elicitor on the biosynthesis of pharmaceutical alkaloid present in marine microalgae
Arthrospira platensis. The experiment as started with addition of salicylic acid (0,
5, 20 and 100) µM concentration to A. platensis suspension culture after 3, 7, 10
and 14 days of culture; followed by estimation of dry weight of biomass and total
alkaloid content. The outcomes revealed that maximum growth was observed
between 8 to 10 days of culture and at 15 days maximum dry weight of biomass
was observed with salicylic acid (5 and 20) µM as well as at 15th day of treatment
maximum content of alkaloid was obtained with 5 µM of salicylic acid. These data
confirmed the importance of salicylic acid on the production of alkaloid in the
marine microalgae (Hadizadeh et al. 2019).
1.4.1.11 Effect of Abiotic Elicitor on Growth of Secondary Metabolites
in Broccoli Plant
Hassini et al. scientifically proved the importance of abiotic elicitors (salicylic acid,
MJ and methionine) on the growth of secondary metabolites present in broccoli
plant. The experiment was started with treating the seeds with potassium chloride,
potassium sulfate and sodium chloride followed by elicited the roots and shoots
with methionine (10 mM), salicylic acid (200 µM) and MJ (100 µM) concentrations. The capacity of roots to conduct water between roots to xylem was measured
by root hydraulic conductivity using root fresh weight and pressure, followed by
assessment of plant defense mechanism using myrosinase activity and total phenolic content present in the plant was also evaluated. The outcomes showed that
maximum dry weight of plant was obtained from roots using potassium sulfate and
from shoots using methionine as elicitor. The root hydraulic conductivity showed
that methionine positively culminated the plant. The myrosinase activity showed
that methionine increase the defense mechanism while sodium chloride, salicylic
acid were suppressed the defense of the plant against insect and herbivores. Two
phenolic acid component as chlorogenic acid and sinapic acid were increased in
1 Elicitor Signal Transduction Leading to the Production of Plant …
11
outcomes revealed that after 5th day of treatment maximum fresh and dry weight of
the callus was observed with activated charcoal and etephon treatments. Also the
amount of anthocyanins were increased with etephon and activated charcoal
treatments. But the amount of extracellular 3-O-glucosyl resveratrol and phenolic
compound were increased with the combination treatment of etephon and high
pressure. These data confirmed the importance of abiotic elicitors on the growth and
accumulation of secondary metabolites present in Vitis vinifera plant (Cai et al.
2011).
1.4.1.10 Effect of Salicylic Acid on Alkaloid Biosynthesis in Marine
Microalgae Arthrospira platensis
Hadizadeh et al. experimentally proved the importance of salicylic acid as abiotic
elicitor on the biosynthesis of pharmaceutical alkaloid present in marine microalgae
Arthrospira platensis. The experiment as started with addition of salicylic acid (0,
5, 20 and 100) µM concentration to A. platensis suspension culture after 3, 7, 10
and 14 days of culture; followed by estimation of dry weight of biomass and total
alkaloid content. The outcomes revealed that maximum growth was observed
between 8 to 10 days of culture and at 15 days maximum dry weight of biomass
was observed with salicylic acid (5 and 20) µM as well as at 15th day of treatment
maximum content of alkaloid was obtained with 5 µM of salicylic acid. These data
confirmed the importance of salicylic acid on the production of alkaloid in the
marine microalgae (Hadizadeh et al. 2019).
1.4.1.11 Effect of Abiotic Elicitor on Growth of Secondary Metabolites
in Broccoli Plant
Hassini et al. scientifically proved the importance of abiotic elicitors (salicylic acid,
MJ and methionine) on the growth of secondary metabolites present in broccoli
plant. The experiment was started with treating the seeds with potassium chloride,
potassium sulfate and sodium chloride followed by elicited the roots and shoots
with methionine (10 mM), salicylic acid (200 µM) and MJ (100 µM) concentrations. The capacity of roots to conduct water between roots to xylem was measured
by root hydraulic conductivity using root fresh weight and pressure, followed by
assessment of plant defense mechanism using myrosinase activity and total phenolic content present in the plant was also evaluated. The outcomes showed that
maximum dry weight of plant was obtained from roots using potassium sulfate and
from shoots using methionine as elicitor. The root hydraulic conductivity showed
that methionine positively culminated the plant. The myrosinase activity showed
that methionine increase the defense mechanism while sodium chloride, salicylic
acid were suppressed the defense of the plant against insect and herbivores. Two
phenolic acid component as chlorogenic acid and sinapic acid were increased in
1 Elicitor Signal Transduction Leading to the Production of Plant …
11