1.4.1.8 Effect of Gamma Radiation on Secondary Metabolites
of Hypericum triquetrifolium Turra
Azeez et al. was scientifically proved the effect of abiotic elicitor (gamma radiation)
on the production of biomass and secondary metabolites (phenolic compounds and
naphtodiantrones) in Hypericum triquetrifolium Turra plant. The experiment was
started with induction of explants (obtained from leaf, stem and root) with indole
acetic acid (IAA) and thidiazuron (synthetic cytokinin) in MS medium followed by
irradiated with gamma rays of 10, 20, 30 and 40 Gy unit; then finally quantify its
average growth index based on the values of callus biomass at initial and final point.
The outcomes revealed that at 10 Gy scale maximum growth index was observed as
well as maximum accumulation of 4-hydroxybenzoic acid, chlorogenic acid and
epicatechin. At 10 Gy of gamma irradiation the accumulation of naphtodiantrones
(hypericina and pseudohypericin) were observed (Fig. 1.4). So it was quite
resemble that 10 Gy scale of gamma rays directly helps to accumulate the principle
secondary metabolites present in the plant (Azeez et al 2017).
1.4.1.9 Effects of Abiotic Elicitors on Secondary Metabolites of Vitis
vinifera Suspension Culture
Cai et al. was experimentally proved the importance of abiotic elicitor (streptomycin, activated charcoal, etephon) and pressure on the growth of secondary
metabolites of Vitis vinifera plant. The experiment was started with the reaction
between plant cell cultures with the above mentioned abiotic elicitors along with a
diverse pressure treatment from (40–50) Mega Pascal unit for a seven days regime.
Here streptomycin was used to reduce the load of contamination, activated charcoal
Fig. 1.4 The morphological characteristics of Hypericum triquetrifolium Turra callus mass
accumulated from leaf explant and irradiated with 10 Gy dose after the third successive subculture
(a). Hypericum triquetrifolium T. callus mass from leaf irradiated with 30 Gy dose and harvested
after the third regular subculture (b). Copyright permission obtained from Azeez et al. @ 2017
Elsevier B.V
10
S. Saha and D. Pal
of Hypericum triquetrifolium Turra
Azeez et al. was scientifically proved the effect of abiotic elicitor (gamma radiation)
on the production of biomass and secondary metabolites (phenolic compounds and
naphtodiantrones) in Hypericum triquetrifolium Turra plant. The experiment was
started with induction of explants (obtained from leaf, stem and root) with indole
acetic acid (IAA) and thidiazuron (synthetic cytokinin) in MS medium followed by
irradiated with gamma rays of 10, 20, 30 and 40 Gy unit; then finally quantify its
average growth index based on the values of callus biomass at initial and final point.
The outcomes revealed that at 10 Gy scale maximum growth index was observed as
well as maximum accumulation of 4-hydroxybenzoic acid, chlorogenic acid and
epicatechin. At 10 Gy of gamma irradiation the accumulation of naphtodiantrones
(hypericina and pseudohypericin) were observed (Fig. 1.4). So it was quite
resemble that 10 Gy scale of gamma rays directly helps to accumulate the principle
secondary metabolites present in the plant (Azeez et al 2017).
1.4.1.9 Effects of Abiotic Elicitors on Secondary Metabolites of Vitis
vinifera Suspension Culture
Cai et al. was experimentally proved the importance of abiotic elicitor (streptomycin, activated charcoal, etephon) and pressure on the growth of secondary
metabolites of Vitis vinifera plant. The experiment was started with the reaction
between plant cell cultures with the above mentioned abiotic elicitors along with a
diverse pressure treatment from (40–50) Mega Pascal unit for a seven days regime.
Here streptomycin was used to reduce the load of contamination, activated charcoal
Fig. 1.4 The morphological characteristics of Hypericum triquetrifolium Turra callus mass
accumulated from leaf explant and irradiated with 10 Gy dose after the third successive subculture
(a). Hypericum triquetrifolium T. callus mass from leaf irradiated with 30 Gy dose and harvested
after the third regular subculture (b). Copyright permission obtained from Azeez et al. @ 2017
Elsevier B.V
10
S. Saha and D. Pal