(Fig. 1.2). After 150 and 157 days, maximum affinin content was observed with
10 mM of salicylic acid and 200 mM of hydrogen peroxide. These data confirmed
the positive effects of abiotic elicitors on chilcaugaue (Parola-Contreras et al. 2020).
1.4.1.5 Effects of Abiotic Elicitor on Hypocrellin a Content in Shiraia
bambusicola
Lu et al. was scientifically proved the effect of abiotic elicitor (lanthum La
3+ ) on the
content of hypocrellin A (anticancer agent mainly against lung adenocarcinoma)
present in Shiraia bambusicola (parasitic fungi present in bamboo). The experiment
was started with mycelium of S. bambusicola and lanthum (La
3+ ) with a concentration range from (0.0–1.4 g/L). The elicited fungus was evaluated by membrane
permeabilization assay with fluorescent dye SYTOX green, generation of reactive
oxygen species using 7-dichloro dihydro fluoresceindiacetate dye followed by
antioxidative activity using NADPH oxidase produce superoxide, superoxide dismutase and catalase enzymes. The outcomes showed that after lanthanum treatment
for 4 h, the hyphae became more strong and intense. The bioactivities showed that
after lanthum treatment the amount of hydrogen peroxide was higher with many
fold increased in antioxidative enzymatic activity. Also vitamin C suppressed the up
regulation of major facilitator superfamily transporter and O-methyltransferase,
polyketide synthase, ATP-binding cassette transporter, O-methyltransferase/
FAD-dependent monooxygenase and FAD/FMN-dependent oxidoreductase
genes. These data correlated with the greater production of hypocrellin A in lanthanum elicitor induced fungi (Lu et al. 2019a).
Fig. 1.2 Typical morphological aspect at day 164 post-transplanting of H. longipes treated with
hydrogen peroxide and salicylic acid. Copyright permission obtained from Parola-Contreras et al.
@ 2020 Elsevier B.V
8
S. Saha and D. Pal
10 mM of salicylic acid and 200 mM of hydrogen peroxide. These data confirmed
the positive effects of abiotic elicitors on chilcaugaue (Parola-Contreras et al. 2020).
1.4.1.5 Effects of Abiotic Elicitor on Hypocrellin a Content in Shiraia
bambusicola
Lu et al. was scientifically proved the effect of abiotic elicitor (lanthum La
3+ ) on the
content of hypocrellin A (anticancer agent mainly against lung adenocarcinoma)
present in Shiraia bambusicola (parasitic fungi present in bamboo). The experiment
was started with mycelium of S. bambusicola and lanthum (La
3+ ) with a concentration range from (0.0–1.4 g/L). The elicited fungus was evaluated by membrane
permeabilization assay with fluorescent dye SYTOX green, generation of reactive
oxygen species using 7-dichloro dihydro fluoresceindiacetate dye followed by
antioxidative activity using NADPH oxidase produce superoxide, superoxide dismutase and catalase enzymes. The outcomes showed that after lanthanum treatment
for 4 h, the hyphae became more strong and intense. The bioactivities showed that
after lanthum treatment the amount of hydrogen peroxide was higher with many
fold increased in antioxidative enzymatic activity. Also vitamin C suppressed the up
regulation of major facilitator superfamily transporter and O-methyltransferase,
polyketide synthase, ATP-binding cassette transporter, O-methyltransferase/
FAD-dependent monooxygenase and FAD/FMN-dependent oxidoreductase
genes. These data correlated with the greater production of hypocrellin A in lanthanum elicitor induced fungi (Lu et al. 2019a).
Fig. 1.2 Typical morphological aspect at day 164 post-transplanting of H. longipes treated with
hydrogen peroxide and salicylic acid. Copyright permission obtained from Parola-Contreras et al.
@ 2020 Elsevier B.V
8
S. Saha and D. Pal