create any remarkable effect and also the different ratios of MJ regulated the plant
secondary metabolites growth depends upon their species characteristics
(Kleinwachter et al. 2015).
1.4.1.3 Effect of Abiotic Elicitor on Triterpenoid Accumulation
in Centella asiatica
Buraphaka et al. was scientifically tested the effects of abiotic elicitor (MJ and
salicylic acid) on the accumulation of triterpenoid in Centella asiatica plant. The
experiment was started with the reaction between different concentrations of MJ
and salicylic acid (1, 2 and 4 mM) with the leaves of the plant under a shaker for
20 min, 40, 60 and 120 min. Then the treated leaves were divided into two groups:
first group for anti-inflammatory was dried at 50 °C whereas another group was
stored at (−) 80 °C for messenger RNA level expression analysis. The outcomes
revealed that after the treatment with salicylic acid (2 mM), the amount of triterpenoid was doubled; whereas treatment with MJ the amount of increased content
was 1.4 times than normal. Also the elicited leaves were remarkably inhibited the
nitric oxide production in lipopolysaccharide induced RAW 264.7 macrophage
cells with increased activity of phenylalanine ammonia lyase, peroxidase and
catalase enzymes. So these data correlated with the positive effect of abiotic elicitor
on the aforementioned plant (Buraphaka and Putalun 2020).
1.4.1.4 Effect of Abiotic Elicitor on Affinin Contents in Heliopsis
longipes (Chilcuague)
Parola-Contreras et al. was experimentally proved the effects of salicylic acid and
hydrogen peroxide as aboiotic elicitor on the amount of affinin content in Heliopsis
longipes (chilcuague). The experiment was started with the treatment of the samplings of chilcuague and salicylic acid (5 and 10 mM) at 150 days after transplanting as well as hydrogen peroxide (200 and 400 mM) at 157 days after
transplanting. Then the elicited plants were evaluated with the enzymatic activity
for superoxide dismutase, catalase, phenylalanine ammonia lyase, valine decarboxylase and also quantified the amount of affinin present in the plant. The outcomes revealed that after 150 days maximum superoxide dismutase, catalase,
phenylalanine ammonia lyase and valine decarboxylase activities were observed
with 10 mM of salicylic acid, 200 mM of hydrogen peroxide, both salicylic acid
and hydrogen peroxide, 200 mM of hydrogen peroxide as abiotic elicitor. After
157 days, maximum superoxide dismutase, catalase, phenylalanine ammonia lyase
were observed with 10 mM of salicylic acid whereas maximum valine decarboxylase activity was observed with 200 mM of hydrogen peroxide elicitor
1 Elicitor Signal Transduction Leading to the Production of Plant …
7
secondary metabolites growth depends upon their species characteristics
(Kleinwachter et al. 2015).
1.4.1.3 Effect of Abiotic Elicitor on Triterpenoid Accumulation
in Centella asiatica
Buraphaka et al. was scientifically tested the effects of abiotic elicitor (MJ and
salicylic acid) on the accumulation of triterpenoid in Centella asiatica plant. The
experiment was started with the reaction between different concentrations of MJ
and salicylic acid (1, 2 and 4 mM) with the leaves of the plant under a shaker for
20 min, 40, 60 and 120 min. Then the treated leaves were divided into two groups:
first group for anti-inflammatory was dried at 50 °C whereas another group was
stored at (−) 80 °C for messenger RNA level expression analysis. The outcomes
revealed that after the treatment with salicylic acid (2 mM), the amount of triterpenoid was doubled; whereas treatment with MJ the amount of increased content
was 1.4 times than normal. Also the elicited leaves were remarkably inhibited the
nitric oxide production in lipopolysaccharide induced RAW 264.7 macrophage
cells with increased activity of phenylalanine ammonia lyase, peroxidase and
catalase enzymes. So these data correlated with the positive effect of abiotic elicitor
on the aforementioned plant (Buraphaka and Putalun 2020).
1.4.1.4 Effect of Abiotic Elicitor on Affinin Contents in Heliopsis
longipes (Chilcuague)
Parola-Contreras et al. was experimentally proved the effects of salicylic acid and
hydrogen peroxide as aboiotic elicitor on the amount of affinin content in Heliopsis
longipes (chilcuague). The experiment was started with the treatment of the samplings of chilcuague and salicylic acid (5 and 10 mM) at 150 days after transplanting as well as hydrogen peroxide (200 and 400 mM) at 157 days after
transplanting. Then the elicited plants were evaluated with the enzymatic activity
for superoxide dismutase, catalase, phenylalanine ammonia lyase, valine decarboxylase and also quantified the amount of affinin present in the plant. The outcomes revealed that after 150 days maximum superoxide dismutase, catalase,
phenylalanine ammonia lyase and valine decarboxylase activities were observed
with 10 mM of salicylic acid, 200 mM of hydrogen peroxide, both salicylic acid
and hydrogen peroxide, 200 mM of hydrogen peroxide as abiotic elicitor. After
157 days, maximum superoxide dismutase, catalase, phenylalanine ammonia lyase
were observed with 10 mM of salicylic acid whereas maximum valine decarboxylase activity was observed with 200 mM of hydrogen peroxide elicitor
1 Elicitor Signal Transduction Leading to the Production of Plant …
7