Chapter 11
Biohydrogen Production from
Lignocellulosic Biomass by Extremely
Halotolerant Bacterial Communities from
a Salt Pan and Salt-Damaged Soil
Dyah Asri Handayani Taroepratjeka, Tsuyoshi Imai,
Prapaipid Chairattanamanokorn, Alissara Reungsang, and Yung-Tse Hung
Contents
1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 413
2 Materials and Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 415
2.1 Seed Microorganisms and Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 415
2.2 Culture Conditions and Experimental Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 415
2.3 Analytical Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 416
2.4 Theoretical Hydrogen Production and Yield . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 416
3 Results and Discussion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 417
3.1 Biohydrogen Production at 3–10% Salinity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 417
3.2 Biohydrogen Production at 15–26% Salinity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 419
3.3 Evaluation of the Bacteria’s Requirement for Chloride Ions in High Salinity
Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 421
D. A. H. Taroepratjeka
Department of Environmental Engineering, Faculty of Civil Engineering and Planning, Institut
Teknologi Nasional, Bandung, Bandung, Jawa Barat, Indonesia
T. Imai (*)
Graduate School of Sciences and Technology for Innovation, Yamaguchi University,
Yamaguchi, Japan
e-mail: imai@yamaguchi-u.ac.jp
P. Chairattanamanokorn
Department of Environmental Technology and Management, Faculty of Environment, Kasetsart
University, Bangkok, Thailand
e-mail: fscippck@ku.ac.th
A. Reungsang
Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen,
Thailand
e-mail: alissara@kku.ac.th
Y.-T. Hung
Civil Engineering Department, Cleveland State University, Cleveland, OH, USA
e-mail: y.hung@csuohio.edu
© Springer Nature Switzerland AG 2021
L. K. Wang, M. -H. S. Wang, Y. -T. Hung, N. K. Shammas (eds.), Integrated Natural
Resources Management, Handbook of Environmental Engineering 20,
https://doi.org/10.1007/978-3-030-55172-8_11
411
Biohydrogen Production from
Lignocellulosic Biomass by Extremely
Halotolerant Bacterial Communities from
a Salt Pan and Salt-Damaged Soil
Dyah Asri Handayani Taroepratjeka, Tsuyoshi Imai,
Prapaipid Chairattanamanokorn, Alissara Reungsang, and Yung-Tse Hung
Contents
1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 413
2 Materials and Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 415
2.1 Seed Microorganisms and Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 415
2.2 Culture Conditions and Experimental Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 415
2.3 Analytical Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 416
2.4 Theoretical Hydrogen Production and Yield . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 416
3 Results and Discussion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 417
3.1 Biohydrogen Production at 3–10% Salinity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 417
3.2 Biohydrogen Production at 15–26% Salinity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 419
3.3 Evaluation of the Bacteria’s Requirement for Chloride Ions in High Salinity
Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 421
D. A. H. Taroepratjeka
Department of Environmental Engineering, Faculty of Civil Engineering and Planning, Institut
Teknologi Nasional, Bandung, Bandung, Jawa Barat, Indonesia
T. Imai (*)
Graduate School of Sciences and Technology for Innovation, Yamaguchi University,
Yamaguchi, Japan
e-mail: imai@yamaguchi-u.ac.jp
P. Chairattanamanokorn
Department of Environmental Technology and Management, Faculty of Environment, Kasetsart
University, Bangkok, Thailand
e-mail: fscippck@ku.ac.th
A. Reungsang
Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen,
Thailand
e-mail: alissara@kku.ac.th
Y.-T. Hung
Civil Engineering Department, Cleveland State University, Cleveland, OH, USA
e-mail: y.hung@csuohio.edu
© Springer Nature Switzerland AG 2021
L. K. Wang, M. -H. S. Wang, Y. -T. Hung, N. K. Shammas (eds.), Integrated Natural
Resources Management, Handbook of Environmental Engineering 20,
https://doi.org/10.1007/978-3-030-55172-8_11
411