4 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 423
Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 425
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 425
Abstract Extremely halotolerant hydrogen-producing bacteria were investigated,
owing to their ability to live in high salinity conditions. Based on this characteristic,
it was hypothesized that extremely halophilic hydrogen-producing bacteria can
tolerate high concentrations of Na
+ ions. To test this hypothesis, we investigated
the characteristics of extremely halotolerant hydrogen-producing bacteria obtained
from salt-damaged soil in Khon Kaen and a commercial salt pan field near Bangkok
(Samut Sakhon), Thailand. Results of this preliminary investigation showed that
hydrogen production under saturated conditions of 26% (6 M) NaCl was possible
after 1 year of acclimatization. The extremely halotolerant hydrogen-producing
bacteria in this research were also confirmed to have a requirement for Cl
À ions
for hydrogen production. Therefore, these extremely halotolerant hydrogenproducing bacteria are suitable for hydrogen production from lignocellulosic
biomass.
Keywords Biohydrogen production · Lignocellulosic biomass · Extremely
halotolerant bacterial communities
Nomenclature
μmol
Micromolar
ATP
Adenosine triphosphate
CaCl 2 Á2H 2 O
Calcium chloride dihydrate
Cl
À
Chloride ion
CO 2
Carbon dioxide
CoCl 2 Á6H 2 O
Cobalt (II) chloride hexahydrate
d
Day
F/M
Food to microorganisms
FeCl 3 Á6H 2 O
Iron (III) chloride hexahydrate
g
Gram
g/L
Gram/liter
h
Hours
H 2
Hydrogen
HAc
Acetic acid
HBu
Butyric acid
HMY
Hydrogen molar yield
K
+
Potassium ion
K 2 HPO 4
Dipotassium phosphate
KCl
Potassium chloride
L
Liter
M
Molar
412
D. A. H. Taroepratjeka et al.