33. Fan Y et al (2018) Characterization of 3-ketoacyl-coA synthase in a nervonic acid producing
oleaginous microalgae Mychonastes afer. Algal
Res 31:225–231
34. Dehesh K, Tai H, Edwards P, Byrne J, Jaworski
JG (2001) Overexpression of 3-ketoacyl-acylcarrier protein synthase IIIs in plants reduces
the rate of lipid synthesis. Plant Physiol
125(2):1103–1114
35. Sun X-M et al (2018) Enhancement of lipid
accumulation in microalgae by metabolic engineering. Biochim Biophys Acta Mol Cell Biol
Lipids
36. Klok A et al (2014) Edible oils from microalgae: insights in TAG accumulation. Trends
Biotechnol 32(10):521–528
37. Shan D et al (2010) GPAT3 and GPAT4 are
regulated by insulin-stimulated phosphorylation and play distinct roles in adipogenesis. J
Lipid Res 51(7):1971–1981. https://doi.org/
10.1194/jlr.M006304
38. Oelkers P et al (2002) The DGA1 gene determines a second triglyceride synthetic pathway
in yeast. J Biol Chem 277(11):8877–8881
39. Cases S et al (1998) Identification of a gene
encoding an acyl CoA: diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis.
Proc Natl Acad Sci 95(22):13018–13023
40. Li J et al (2014) Choreography of transcriptomes and lipidomes of nannochloropsis
reveals the mechanisms of oil synthesis in
microalgae. Plant Cell 26(4):1645–1665
41. Niu Y-F et al (2013) Improvement of neutral
lipid and polyunsaturated fatty acid biosynthesis by overexpressing a type 2 diacylglycerol
acyltransferase in marine diatom Phaeodactylum
tricornutum.
Mar
Drugs
11
(11):4558–4569
42. Chen CY et al (2016) Expression of type 2 diacylglycerol acyltransferse gene DGTT1 from
Chlamydomonas reinhardtii enhances lipid
production in Scenedesmus obliquus. Biotechnol J 11(3):336–344
43. Hung C-H et al (2013) Functional study of
diacylglycerol acyltransferase type 2 family in
Chlamydomonas reinhardtii. FEBS Lett 587
(15):2364–2370
44. La Russa M et al (2012) Functional analysis of
three type-2 DGAT homologue genes for triacylglycerol production in the green microalga
Chlamydomonas reinhardtii. J Biotechnol 162
(1):13–20
45. Boyle NR, Morgan JA (2009) Flux balance
analysis of primary metabolism in Chlamydomonas reinhardtii. BMC Syst Biol 3(1):4
46. Ma Y-H et al (2014) Antisense knockdown of
pyruvate dehydrogenase kinase promotes the
neutral lipid accumulation in the diatom
Phaeodactylum tricornutum. Microb Cell
Fact 13(1):100
47. Xue J et al (2015) Genetic improvement of the
microalga Phaeodactylum tricornutum for
boosting neutral lipid accumulation. Metab
Eng 27:1–9
48. Guan Y et al (2004) Two-stage photobiological production of hydrogen by marine
green alga Platymonas subcordiformis. Biochem Eng J 19(1):69–73
49. Melis A, Melnicki MR (2006) Integrated
biological hydrogen production. Int J Hydrogen Energy 31(11):1563–1573
50. Eroglu E, Melis A (2016) Microalgal hydrogen
production research. Int J Hydrogen Energy
41(30):12772–12798
51. Gimpel JA et al (2013) Advances in microalgae
engineering and synthetic biology applications
for biofuel production. Curr Opin Chem Biol
17(3):489–495
52. Shuba ES, Kifle D (2018) Microalgae to biofuels: ‘Promising’ alternative and renewable
energy, review. Renew Sustain Energy Rev
81:743–755
53. Beer LL et al (2009) Engineering algae for
biohydrogen and biofuel production. Curr
Opin Biotechnol 20(3):264–271
54. Khetkorn W et al (2017) Microalgal hydrogen
production—a review. Bioresour Technol
243:1194–1206
55. Surzycki R et al (2007) Potential for hydrogen
production with inducible chloroplast gene
expression in Chlamydomonas. Proc Natl
Acad Sci 104(44):17548–17553
56. Dubini A, Ghirardi ML (2015) Engineering
photosynthetic organisms for the production
of biohydrogen. Photosynth Res 123
(3):241–253
57. Esquı ´vel MG et al (2011) Efficient H2 production via Chlamydomonas reinhardtii. Trends
Biotechnol 29(12):595–600
58. Lee H-S, Vermaas WF, Rittmann BE (2010)
Biological hydrogen production: prospects
and challenges. Trends Biotechnol 28
(5):262–271
59. Specht E, Miyake-Stoner S, Mayfield S (2010)
Micro-algae come of age as a platform for
recombinant protein production. Biotechnol
Lett 32(10):1373–1383
60. Heydarizadeh P et al (2013) Plastids of marine
phytoplankton produce bioactive pigments and
lipids. Mar Drugs 11(9):3425–3471
61. Terashima M, Specht M, Hippler M (2011)
The chloroplast proteome: a survey from the
Chlamydomonas reinhardtii perspective with a
focus on distinctive features. Curr Genet 57
(3):151–168
170
Mohammad Pooya Naghshbandi et al.
oleaginous microalgae Mychonastes afer. Algal
Res 31:225–231
34. Dehesh K, Tai H, Edwards P, Byrne J, Jaworski
JG (2001) Overexpression of 3-ketoacyl-acylcarrier protein synthase IIIs in plants reduces
the rate of lipid synthesis. Plant Physiol
125(2):1103–1114
35. Sun X-M et al (2018) Enhancement of lipid
accumulation in microalgae by metabolic engineering. Biochim Biophys Acta Mol Cell Biol
Lipids
36. Klok A et al (2014) Edible oils from microalgae: insights in TAG accumulation. Trends
Biotechnol 32(10):521–528
37. Shan D et al (2010) GPAT3 and GPAT4 are
regulated by insulin-stimulated phosphorylation and play distinct roles in adipogenesis. J
Lipid Res 51(7):1971–1981. https://doi.org/
10.1194/jlr.M006304
38. Oelkers P et al (2002) The DGA1 gene determines a second triglyceride synthetic pathway
in yeast. J Biol Chem 277(11):8877–8881
39. Cases S et al (1998) Identification of a gene
encoding an acyl CoA: diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis.
Proc Natl Acad Sci 95(22):13018–13023
40. Li J et al (2014) Choreography of transcriptomes and lipidomes of nannochloropsis
reveals the mechanisms of oil synthesis in
microalgae. Plant Cell 26(4):1645–1665
41. Niu Y-F et al (2013) Improvement of neutral
lipid and polyunsaturated fatty acid biosynthesis by overexpressing a type 2 diacylglycerol
acyltransferase in marine diatom Phaeodactylum
tricornutum.
Mar
Drugs
11
(11):4558–4569
42. Chen CY et al (2016) Expression of type 2 diacylglycerol acyltransferse gene DGTT1 from
Chlamydomonas reinhardtii enhances lipid
production in Scenedesmus obliquus. Biotechnol J 11(3):336–344
43. Hung C-H et al (2013) Functional study of
diacylglycerol acyltransferase type 2 family in
Chlamydomonas reinhardtii. FEBS Lett 587
(15):2364–2370
44. La Russa M et al (2012) Functional analysis of
three type-2 DGAT homologue genes for triacylglycerol production in the green microalga
Chlamydomonas reinhardtii. J Biotechnol 162
(1):13–20
45. Boyle NR, Morgan JA (2009) Flux balance
analysis of primary metabolism in Chlamydomonas reinhardtii. BMC Syst Biol 3(1):4
46. Ma Y-H et al (2014) Antisense knockdown of
pyruvate dehydrogenase kinase promotes the
neutral lipid accumulation in the diatom
Phaeodactylum tricornutum. Microb Cell
Fact 13(1):100
47. Xue J et al (2015) Genetic improvement of the
microalga Phaeodactylum tricornutum for
boosting neutral lipid accumulation. Metab
Eng 27:1–9
48. Guan Y et al (2004) Two-stage photobiological production of hydrogen by marine
green alga Platymonas subcordiformis. Biochem Eng J 19(1):69–73
49. Melis A, Melnicki MR (2006) Integrated
biological hydrogen production. Int J Hydrogen Energy 31(11):1563–1573
50. Eroglu E, Melis A (2016) Microalgal hydrogen
production research. Int J Hydrogen Energy
41(30):12772–12798
51. Gimpel JA et al (2013) Advances in microalgae
engineering and synthetic biology applications
for biofuel production. Curr Opin Chem Biol
17(3):489–495
52. Shuba ES, Kifle D (2018) Microalgae to biofuels: ‘Promising’ alternative and renewable
energy, review. Renew Sustain Energy Rev
81:743–755
53. Beer LL et al (2009) Engineering algae for
biohydrogen and biofuel production. Curr
Opin Biotechnol 20(3):264–271
54. Khetkorn W et al (2017) Microalgal hydrogen
production—a review. Bioresour Technol
243:1194–1206
55. Surzycki R et al (2007) Potential for hydrogen
production with inducible chloroplast gene
expression in Chlamydomonas. Proc Natl
Acad Sci 104(44):17548–17553
56. Dubini A, Ghirardi ML (2015) Engineering
photosynthetic organisms for the production
of biohydrogen. Photosynth Res 123
(3):241–253
57. Esquı ´vel MG et al (2011) Efficient H2 production via Chlamydomonas reinhardtii. Trends
Biotechnol 29(12):595–600
58. Lee H-S, Vermaas WF, Rittmann BE (2010)
Biological hydrogen production: prospects
and challenges. Trends Biotechnol 28
(5):262–271
59. Specht E, Miyake-Stoner S, Mayfield S (2010)
Micro-algae come of age as a platform for
recombinant protein production. Biotechnol
Lett 32(10):1373–1383
60. Heydarizadeh P et al (2013) Plastids of marine
phytoplankton produce bioactive pigments and
lipids. Mar Drugs 11(9):3425–3471
61. Terashima M, Specht M, Hippler M (2011)
The chloroplast proteome: a survey from the
Chlamydomonas reinhardtii perspective with a
focus on distinctive features. Curr Genet 57
(3):151–168
170
Mohammad Pooya Naghshbandi et al.