62. Coragliotti AT et al (2011) Molecular factors
affecting the accumulation of recombinant
proteins in the Chlamydomonas reinhardtii
chloroplast. Mol Biotechnol 48(1):60–75
63. Rasala BA, Mayfield SP (2015) Photosynthetic
biomanufacturing in green algae; production
of recombinant proteins for industrial, nutritional, and medical uses. Photosynth Res 123
(3):227–239
64. Cerutti H et al (2011) RNA-mediated silencing in algae: biological roles and tools for the
analysis of gene function. Eukaryot Cell 10
(9):1164–1172. https://doi.org/10.1128/
EC.05106-11
65. Molnar A et al (2009) Highly specific gene
silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii. Plant J
58(1):165–174
66. Johanningmeier U, Fischer D (2010) Perspective for the use of genetic transformants in
order to enhance the synthesis of the desired
metabolites: engineering chloroplasts of microalgae for the production of bioactive compounds. In: Bio-farms for nutraceuticals.
Springer, New York, pp 144–151
67. Purton S et al (2013) Genetic engineering of
algal chloroplasts: progress and prospects. Russ
J Plant Physiol 60(4):491–499
68. Anand V et al (2017) Proteomic approaches in
microalgae: perspectives and applications.
3 Biotech 7(3):197
69. Banerjee C, Singh PK, Shukla P (2016) Microalgal bioengineering for sustainable energy
development: recent transgenesis and metabolic engineering strategies. Biotechnol J 11
(3):303–314
70. Kasai Y et al (2015) Construction of a selfcloning system in the unicellular green alga
Pseudochoricystis ellipsoidea. Biotechnol Biofuels 8(1):94
71. Shin S-E et al (2016) CRISPR/Cas9-induced
knockout and knock-in mutations in Chlamydomonas reinhardtii. Sci Rep 6:27810
72. Sizova I et al (2013) Nuclear gene targeting in
Chlamydomonas using engineered zinc-finger
nucleases. Plant J 73(5):873–882
73. Andrianantoandro E et al (2006) Synthetic
biology: new engineering rules for an emerging
discipline. Mol Syst Biol 2(1)
74. Bashir KMI et al (2016) Microalgae engineering toolbox: selectable and screenable markers.
Biotechnol Bioprocess Eng 21(2):224–235
75. Jang Y-S et al (2012) Engineering of microorganisms for the production of biofuels and
perspectives based on systems metabolic engineering approaches. Biotechnol Adv 30
(5):989–1000
76. Thiele I, Palsson BØ (2010) A protocol for
generating a high-quality genome-scale metabolic reconstruction. Nat Protoc 5(1):93
77. Chen JW et al (2017) Identification of a malonyl CoA-acyl carrier protein transacylase and
its regulatory role in fatty acid biosynthesis in
oleaginous microalga Nannochloropsis oceanica.
Biotechnol
Appl
Biochem
64
(5):620–626
78. Li Z et al (2018) Overexpression of malonylCoA: ACP transacylase in Schizochytrium
sp. to improve polyunsaturated fatty acid production.
J
Agric
Food
Chem
66
(21):5382–5391
79. Balamurugan S et al (2017) Occurrence of
plastidial triacylglycerol synthesis and the
potential regulatory role of AGPAT in the
model diatom Phaeodactylum tricornutum.
Biotechnol Biofuels 10(1):97
80. Yamaoka Y et al (2016) Identification of a
Chlamydomonas plastidial 2-lysophosphatidic
acid acyltransferase and its use to engineer
microalgae with increased oil content. Plant
Biotechnol J 14(11):2158–2167
81. Zou L-G et al (2018) High-efficiency promoter-driven coordinated regulation of multiple metabolic nodes elevates lipid accumulation
in the model microalga Phaeodactylum tricornutum. Microb Cell Fact 17(1):54
82. Niu Y-F et al (2016) Molecular characterization of a glycerol-3-phosphate acyltransferase
reveals key features essential for triacylglycerol
production in Phaeodactylum tricornutum.
Biotechnol Biofuels 9(1):60
83. Iskandarov U et al (2016) Cloning and characterization of a GPAT-like gene from the microalga Lobosphaera incisa (Trebouxiophyceae):
overexpression in Chlamydomonas reinhardtii
enhances TAG production. J Appl Phycol 28
(2):907–919
84. Wei H et al (2017) A type-I diacylglycerol acyltransferase modulates triacylglycerol biosynthesis and fatty acid composition in the
oleaginous microalga, Nannochloropsis oceanica. Biotechnol Biofuels 10(1):174
85. Iwai M et al (2014) Enhancement of extraplastidic oil synthesis in C hlamydomonas reinhardtii using a type-2 diacylglycerol
acyltransferase with a phosphorus starvation–inducible promoter. Plant Biotechnol J 12
(6):808–819
86. Zienkiewicz K et al (2017) Nannochloropsis, a
rich source of diacylglycerol acyltransferases for
engineering of triacylglycerol content in different hosts. Biotechnol Biofuels 10(1):8
87. Li D-W et al (2016) A type 2 diacylglycerol
acyltransferase accelerates the triacylglycerol
Metabolic Engineering of Microalgae
171
affecting the accumulation of recombinant
proteins in the Chlamydomonas reinhardtii
chloroplast. Mol Biotechnol 48(1):60–75
63. Rasala BA, Mayfield SP (2015) Photosynthetic
biomanufacturing in green algae; production
of recombinant proteins for industrial, nutritional, and medical uses. Photosynth Res 123
(3):227–239
64. Cerutti H et al (2011) RNA-mediated silencing in algae: biological roles and tools for the
analysis of gene function. Eukaryot Cell 10
(9):1164–1172. https://doi.org/10.1128/
EC.05106-11
65. Molnar A et al (2009) Highly specific gene
silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii. Plant J
58(1):165–174
66. Johanningmeier U, Fischer D (2010) Perspective for the use of genetic transformants in
order to enhance the synthesis of the desired
metabolites: engineering chloroplasts of microalgae for the production of bioactive compounds. In: Bio-farms for nutraceuticals.
Springer, New York, pp 144–151
67. Purton S et al (2013) Genetic engineering of
algal chloroplasts: progress and prospects. Russ
J Plant Physiol 60(4):491–499
68. Anand V et al (2017) Proteomic approaches in
microalgae: perspectives and applications.
3 Biotech 7(3):197
69. Banerjee C, Singh PK, Shukla P (2016) Microalgal bioengineering for sustainable energy
development: recent transgenesis and metabolic engineering strategies. Biotechnol J 11
(3):303–314
70. Kasai Y et al (2015) Construction of a selfcloning system in the unicellular green alga
Pseudochoricystis ellipsoidea. Biotechnol Biofuels 8(1):94
71. Shin S-E et al (2016) CRISPR/Cas9-induced
knockout and knock-in mutations in Chlamydomonas reinhardtii. Sci Rep 6:27810
72. Sizova I et al (2013) Nuclear gene targeting in
Chlamydomonas using engineered zinc-finger
nucleases. Plant J 73(5):873–882
73. Andrianantoandro E et al (2006) Synthetic
biology: new engineering rules for an emerging
discipline. Mol Syst Biol 2(1)
74. Bashir KMI et al (2016) Microalgae engineering toolbox: selectable and screenable markers.
Biotechnol Bioprocess Eng 21(2):224–235
75. Jang Y-S et al (2012) Engineering of microorganisms for the production of biofuels and
perspectives based on systems metabolic engineering approaches. Biotechnol Adv 30
(5):989–1000
76. Thiele I, Palsson BØ (2010) A protocol for
generating a high-quality genome-scale metabolic reconstruction. Nat Protoc 5(1):93
77. Chen JW et al (2017) Identification of a malonyl CoA-acyl carrier protein transacylase and
its regulatory role in fatty acid biosynthesis in
oleaginous microalga Nannochloropsis oceanica.
Biotechnol
Appl
Biochem
64
(5):620–626
78. Li Z et al (2018) Overexpression of malonylCoA: ACP transacylase in Schizochytrium
sp. to improve polyunsaturated fatty acid production.
J
Agric
Food
Chem
66
(21):5382–5391
79. Balamurugan S et al (2017) Occurrence of
plastidial triacylglycerol synthesis and the
potential regulatory role of AGPAT in the
model diatom Phaeodactylum tricornutum.
Biotechnol Biofuels 10(1):97
80. Yamaoka Y et al (2016) Identification of a
Chlamydomonas plastidial 2-lysophosphatidic
acid acyltransferase and its use to engineer
microalgae with increased oil content. Plant
Biotechnol J 14(11):2158–2167
81. Zou L-G et al (2018) High-efficiency promoter-driven coordinated regulation of multiple metabolic nodes elevates lipid accumulation
in the model microalga Phaeodactylum tricornutum. Microb Cell Fact 17(1):54
82. Niu Y-F et al (2016) Molecular characterization of a glycerol-3-phosphate acyltransferase
reveals key features essential for triacylglycerol
production in Phaeodactylum tricornutum.
Biotechnol Biofuels 9(1):60
83. Iskandarov U et al (2016) Cloning and characterization of a GPAT-like gene from the microalga Lobosphaera incisa (Trebouxiophyceae):
overexpression in Chlamydomonas reinhardtii
enhances TAG production. J Appl Phycol 28
(2):907–919
84. Wei H et al (2017) A type-I diacylglycerol acyltransferase modulates triacylglycerol biosynthesis and fatty acid composition in the
oleaginous microalga, Nannochloropsis oceanica. Biotechnol Biofuels 10(1):174
85. Iwai M et al (2014) Enhancement of extraplastidic oil synthesis in C hlamydomonas reinhardtii using a type-2 diacylglycerol
acyltransferase with a phosphorus starvation–inducible promoter. Plant Biotechnol J 12
(6):808–819
86. Zienkiewicz K et al (2017) Nannochloropsis, a
rich source of diacylglycerol acyltransferases for
engineering of triacylglycerol content in different hosts. Biotechnol Biofuels 10(1):8
87. Li D-W et al (2016) A type 2 diacylglycerol
acyltransferase accelerates the triacylglycerol
Metabolic Engineering of Microalgae
171