Alnus
glutinosa
Secondary treated
wastewater
River water
or secondary
treated
wastewater
2.1 cm
2 leaves at the
time every
7 days
4 weeks
Leaves:
Before: 60
C for 24 h
After: 60
C for 24 h
Organisms:
After: 60
C for 24 h
C
¼
Lb
 k
ð ÞÀLe
½
Š
gÂt
C
¼ leaf mass consumed
L
b ¼ initial dry mass of the leaf
disc (mg)
L
e ¼
final dry mass of the leaf
disc (mg)
g
¼ animal
0
s dry mass (mg)
t
¼ feeding time (days)
k
¼ leaf change correction factor
k
¼
P
L
ob ÀLoe
L
ob
h
i
n
L
ob ¼ initial dry mass of control
discs (mg)
L
oe ¼
final dry mass of control leaf
discs (mg)
n
¼ number of replicates
Bundschuh
et al.
(2011b)
Alnus
glutinosa
Antibiotics
(added during the
conditioning process and in the
water with the
Gammarids)
River and tap
water
mixture
1.1 cm
2 leaves at the
time
24 days
Leaves:
Before: 60
C for 24 h
After: 60
C for 24 h
Organisms:
After: 60
C for 24 h
C
¼
La
 1Àk
ð
Þ À Lb
½
Š
gÂt
C
¼ leaf mass consumed
L
a ¼ initial dry mass of the leaf
disc (mg)
L
b ¼
final dry mass of the leaf
disc (mg)
g
¼ animal
’
s dry mass (mg)
t
¼ feeding time (days)
k
¼
P
Lc
ÀL
d
Lc
½
Š
n
L
c ¼ initial dry mass of control
discs (mg)
L
d ¼
final dry mass of control leaf
discs (mg)
n
¼ number of replicates
Bundschuh
et al. (2017)
Alnus
glutinosa
In situ feeding
assay
River water
1.7 cm
4 leaf discs per
cage
6 days
Leaves:
Before:
After: 60
C for 48 h
Organisms:
After: 60
C for 48 h
C
¼
L1
ÂC1
ð
Þ À L2
WÂT
C
¼ feeding rate
L
1 ¼ initial leaves dry weight (mg)
L
2 ¼
final leaves dry weight (mg)
W
¼ organisms dry weight (mg)
C
1 ¼ leaf weight change correction
factor
T
¼ feeding time (days)
Crane and
Maltby
(1991)
(continued)
Feeding Behavioural Studies with Freshwater Gammarus spp.: The. . .
27