CHAPTER II
MATERIALS AND METHODS
40
Figure 37: chloroform
Figure 38: the result of adding
chloroform to the supernatant
To this mix, 2 ml of 2N hydrochloric acid (HCl) were added, and then was heated in a water
bath for 2 hours at 80°C.
The mixture was once again centrifuged at 4000 rpm for 20 minutes, followed by 5 ml of
chloroform added to the supernatant.
The chloroform mixture was left overnight at 28°C on a stirring water bath to extract the PHB.
The mixture was centrifuged one more time at 4000 rpm for 20 minutes, extracted with 0.1 ml
of chloroform and dried at 40°C.
Figure 39: stirring water bath
Figure 36: water bath set at 80°C
Figure 35: the addition of HCl 2N to
the suspension