CHAPTER II
MATERIALS AND METHODS
Figure 34: the mixture after vortex
Figure 29: the collected spirulina culture
Figure 30: centrifugation
Figure 31: the tubes after centrifugation
Figure 32: an oven set at 100°C
• Balance
• Vortex mixer
• Water bath
• Stirring water bath
• Laboratory tubes
• Microscope
• Microscope slides
2.2 Method
Initially, 10 ml of Spirulina platensis culture were collected and centrifuged at 4000 rpm for 30
minutes to separate the cells.
Then, the pellet was placed in the oven and dried at 100°C for 24 hours.
The dried cell pellet was suspended in sterile water, homogenized, and then passed through
vortex mixing.
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Figure 33: vortex mixing of
the dried pellet and water