CHAPTER II
MATERIALS AND METHODS
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Figure 27: 10 L of LMK medium
prepared
Figure 28: the seeded culture inside the aquarium
After observing the results, additional medium was prepared and used in several seedings. The
spirulina culture expanded in volume, and its characteristic blue-green color became evident.
2 Extraction of Polyhydroxybutyrate (PHB)
The extraction of PHB from Spirulina platensis was carried out using a chemical method.
2.1 Materials
• Spirulina platensis culture
• Chemical reactives: 2N HCL, Chloroform.
• Microcentrifuge
• Microcentrifuge tubes
• Oven
Figure 26: Spirulina culture after seeding