CHAPTER II
MATERIALS AND METHODS
Figure 17: Adding 2 ml of
nutrient medium
Figure 18: mixing spirulina culture
Figure 13: pH meter
Figure 14: Thermometer
Figure 15: NaOH
Figure 16: HCl
To ensure uniform distribution of nutrients and CO2, the culture was mixed using a spatula at
least three times a day. Furthermore, to sustain the spirulina culture and support its continuous
growth, a 2 ml of nutrient medium was added every 48 hours.
Spirulina was reseeded weekly in fresh K Marine culture medium to increase its volume. To
maintain proper conditions/concentration, the culture medium volume was adjusted to a 4:1
ratio if the spirulina was too concentrated and a 3:1 ratio if it was less concentrated.
36
Figure 20: pouring the
spirulina into a fresh medium
Figure 19: Concentrated spirulina culture
with a fresh culture medium
Figure 21: Results after reseeding