CHAPTER II
MATERIALS AND METHODS
Figure 12: spirulina platensis culture inside the aquarium
Figure 11: the freshly seeded spirulina culture
The living culture was first seeded and transferred into 1L and 2L jars, which were then placed
inside the aquarium. The seeding process involved adding 13 g of culture medium powder to
1L of water, mixing well in a 2L container, and then adding 250 ml of spirulina culture to the
mixture.
Figure 8: weighing of
medium powder
Figure 9: mixing medium with water
Figure 10: adding spirulina to the medium
The culture was kept under continuous and daily monitoring of pH levels and temperature.
The water temperature was kept at 30°C with a thermal resistance, and the pH level of the
spirulina was maintained at 9-10 using NaOH or HCl.
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