76
3 Bio-augmented Composting of Organic Wastes in Villages and Towns
Fig. 3.7 Changes of pH value and Ec in the composting process through different treatments (Song
et al. 2018)
conductivity gradually reduced. The conductivity of the control group constantly
rose. Except for the inoculated group, the conductivities of the other two treatment
groups were both higher than 2.6 ms/cm.
3.3.2.2 Composting Mechanism for Rapid Humification by Adding
Acidification-Resistant Complex Microbial Inoculants
Acetic acid mainly came from pyruvic acid, which generated acetic acid through
glycolysis based on pyruvate dehydrogenase and aldehyde dehydrogenase. By using
acetyl-coenzyme A synthetase, acetic acid produced acetyl coenzyme A, which was
finally subjected to tricarboxylic acid (TCA) cycle and further utilised through glyoxylate cycle branch. The biochemical reactions triggered by acetyl-coenzyme A
synthetase and malate synthase both appeared in the direction of degradation of
acetic acid. Compared with the control group, the diversities of microorganisms
generating acetyl-coenzyme A synthetase and malate synthase in the inoculated
group both greatly grew. It revealed that inoculating acidification-resistant complex
microbial inoculants can significantly improve four key enzymes (A, B, C and D) in
the metabolic pathways of acetic acid. Enzyme A denotes pyruvate dehydrogenase,
which was generated from Staphylococcus spp., Pseudomonas spp. and Bacillus
spp. in the inoculated group. In the alkalified group, enzyme A was generated by
Escherichia spp., Pseudomonas spp., Bacillus spp. and Mycobacterium spp., while it
was produced by Mycobacterium spp. in the CK group. Enzyme B refers to aldehyde
dehydrogenase, which was generated by Rhodococcus spp. both in inoculated and
alkalified groups while no enzyme B was detected in the CK group. In the metabolic
pathways of acetic acid, the biochemical reactions facilitated by enzymes A and B
both occurred at the side where acetic acid was generated. Compared with the control group, the diversities of microorganisms generating enzymes A and B both rose
in inoculated and alkalified groups. However, compared with alkalified group, the
diversity of microorganisms generating enzymes A and B in the inoculated group was
3 Bio-augmented Composting of Organic Wastes in Villages and Towns
Fig. 3.7 Changes of pH value and Ec in the composting process through different treatments (Song
et al. 2018)
conductivity gradually reduced. The conductivity of the control group constantly
rose. Except for the inoculated group, the conductivities of the other two treatment
groups were both higher than 2.6 ms/cm.
3.3.2.2 Composting Mechanism for Rapid Humification by Adding
Acidification-Resistant Complex Microbial Inoculants
Acetic acid mainly came from pyruvic acid, which generated acetic acid through
glycolysis based on pyruvate dehydrogenase and aldehyde dehydrogenase. By using
acetyl-coenzyme A synthetase, acetic acid produced acetyl coenzyme A, which was
finally subjected to tricarboxylic acid (TCA) cycle and further utilised through glyoxylate cycle branch. The biochemical reactions triggered by acetyl-coenzyme A
synthetase and malate synthase both appeared in the direction of degradation of
acetic acid. Compared with the control group, the diversities of microorganisms
generating acetyl-coenzyme A synthetase and malate synthase in the inoculated
group both greatly grew. It revealed that inoculating acidification-resistant complex
microbial inoculants can significantly improve four key enzymes (A, B, C and D) in
the metabolic pathways of acetic acid. Enzyme A denotes pyruvate dehydrogenase,
which was generated from Staphylococcus spp., Pseudomonas spp. and Bacillus
spp. in the inoculated group. In the alkalified group, enzyme A was generated by
Escherichia spp., Pseudomonas spp., Bacillus spp. and Mycobacterium spp., while it
was produced by Mycobacterium spp. in the CK group. Enzyme B refers to aldehyde
dehydrogenase, which was generated by Rhodococcus spp. both in inoculated and
alkalified groups while no enzyme B was detected in the CK group. In the metabolic
pathways of acetic acid, the biochemical reactions facilitated by enzymes A and B
both occurred at the side where acetic acid was generated. Compared with the control group, the diversities of microorganisms generating enzymes A and B both rose
in inoculated and alkalified groups. However, compared with alkalified group, the
diversity of microorganisms generating enzymes A and B in the inoculated group was