74
3 Bio-augmented Composting of Organic Wastes in Villages and Towns
Fig. 3.5 The interaction of microbial communities in microbial agent and indigenous communities
3.3.2 Composing Technology for Rapid Humification
of Kitchen Wastes
3.3.2.1 Experimental Design and Implementation
In the early stage of composting of kitchen wastes, material acidification can inhibit
the activity of microorganisms to thus further result in long period and poor fertilizer
efficiency of the composting of kitchen wastes. In order to solve the problem, the
bio-augmented composting technology for oriented humification of kitchen wastes
was explored by utilizing acidification-resistant complex microbial inoculants in
order to realize composting for rapid humification of kitchen wastes. The kitchen
wastes were manually mixed with bran, with the C/N ratio of the compost of 25.4
and the moisture content of 60.7%. Three experimental groups were set, including an
inoculated group, an alkalified group and a control group. For the inoculated group,
1.25 mL of bacterial liquid of acidification-resistant complex microbial inoculants at
exponential phase was added to per kilometer of dry raw materials of compost (concentration of microbial inoculants was 1 × 10
8 CFU mL
−1 ). For alkalified group,
chemical buffers of 0.05 M MgO and 0.1 M K 2 HPO 4 per kilometer of dry raw materials were added. The natural compost without the addition of microbial inoculants
and buffers was taken as the control group. In the composting process, the ventilator
capacity was controlled as 0.5 L min
−1 kg
−1 and also the change of temperature was
monitored in real time. Samples were taken after the composting lasted for 0, 3, 8,
13, 19, 35 and 47 days, respectively. Before each sampling, the compost materials
were manually mixed. The multiple samples collected at the different depths in pile
centre were taken to make into a sample (about 200 g), which was stored at −20 °C
for subsequent utilization.
3 Bio-augmented Composting of Organic Wastes in Villages and Towns
Fig. 3.5 The interaction of microbial communities in microbial agent and indigenous communities
3.3.2 Composing Technology for Rapid Humification
of Kitchen Wastes
3.3.2.1 Experimental Design and Implementation
In the early stage of composting of kitchen wastes, material acidification can inhibit
the activity of microorganisms to thus further result in long period and poor fertilizer
efficiency of the composting of kitchen wastes. In order to solve the problem, the
bio-augmented composting technology for oriented humification of kitchen wastes
was explored by utilizing acidification-resistant complex microbial inoculants in
order to realize composting for rapid humification of kitchen wastes. The kitchen
wastes were manually mixed with bran, with the C/N ratio of the compost of 25.4
and the moisture content of 60.7%. Three experimental groups were set, including an
inoculated group, an alkalified group and a control group. For the inoculated group,
1.25 mL of bacterial liquid of acidification-resistant complex microbial inoculants at
exponential phase was added to per kilometer of dry raw materials of compost (concentration of microbial inoculants was 1 × 10
8 CFU mL
−1 ). For alkalified group,
chemical buffers of 0.05 M MgO and 0.1 M K 2 HPO 4 per kilometer of dry raw materials were added. The natural compost without the addition of microbial inoculants
and buffers was taken as the control group. In the composting process, the ventilator
capacity was controlled as 0.5 L min
−1 kg
−1 and also the change of temperature was
monitored in real time. Samples were taken after the composting lasted for 0, 3, 8,
13, 19, 35 and 47 days, respectively. Before each sampling, the compost materials
were manually mixed. The multiple samples collected at the different depths in pile
centre were taken to make into a sample (about 200 g), which was stored at −20 °C
for subsequent utilization.