Result and discussion
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4- Protein identification test
The aim of this study was to identify the presence or absence of proteins in the tested samples,
namely chitin, chitosan, and egg white solutions. The micro biuret method was utilized as a
qualitative protein identification technique. Where the objective of this analysis was solely
focused on identifying protein presence, and no attempt was made to estimate protein
concentrations in the samples.
The results of the micro biuret protein identification test indicated the presence of proteins in
both the chitin and egg white solutions, as evidenced by the development of a purple color. This
aligns with expectations, as egg white is known to be a rich source of proteins. However, the
chitosan solution yielded a negative result with the appearance of a blue color, indicating the
absence or very low levels of detectable proteins.
This supports the notion that the chitosan extraction process, involving deacetylation and
thorough cleaning, effectively removed or significantly reduced the protein content. The
positive result obtained for the egg white solution, which served as a reference, confirms the
reliability of the micro biuret method for protein detection.
Figure 50:Protein identification test