41
9. Fourier transform infrared spectroscopy (FTIR)
FTIR spectra of chitin and chitosan samples were recorded using FTIR spectrophotometer
(Perkin Elmer) Model: spectrum two, in the range of 400–4000 cm−1.
10. Degree of deacetylation
The FTIR spectra of chitosan were obtained using an IR Instrument, with a frequency range
of 4000–400 cm−1.
The degree of deacetylation (DD) of the chitosan samples was calculated using the method
proposed by (SABNIS and BLOCK, 1997)
6
í µí±«í µí±«í µí±¨ % = 97.67 − [26.486 × (
í µí±¨1655
í µí±¨3450
)]
9. Fourier transform infrared spectroscopy (FTIR)
FTIR spectra of chitin and chitosan samples were recorded using FTIR spectrophotometer
(Perkin Elmer) Model: spectrum two, in the range of 400–4000 cm−1.
10. Degree of deacetylation
The FTIR spectra of chitosan were obtained using an IR Instrument, with a frequency range
of 4000–400 cm−1.
The degree of deacetylation (DD) of the chitosan samples was calculated using the method
proposed by (SABNIS and BLOCK, 1997)
6
í µí±«í µí±«í µí±¨ % = 97.67 − [26.486 × (
í µí±¨1655
í µí±¨3450
)]