40
➢ Pipettes
Procedure :
➢ 1% Chitosan solution is prepared by dissolving 0.2g of chitosan powder in 20 ml of
purified water
➢ 1% chitin solution is prepared by dissolving 0.2g of chitin powder in 20 ml of purified
water
➢ Protein reference solution is prepared by diluting fresh egg white with same amount of
purified water
➢ Using a pipette, add 1-5 ml of biuret reagent to each tube and mix well
➢ Allow the test tubes to stand for 5-10 minutes at room temperature.
➢ Observe any color change, The appearance of a violet color indicates the presence of
protein in the sample.
6. Solubility
A dissolution experiment was conducted by introducing a predetermined quantity of chitosan
powder into a 1% acetic acid solution. The solution was subjected to stirring for an extended
period of time, specifically overnight, at ambient temperature. (SULARSIH et al., 2012)
The purpose of this prolonged stirring was to facilitate the dissolution process and ensure the
complete integration of the chitosan powder within the acetic acid solution. This experimental
procedure aimed to obtain a homogeneous chitosan solution suitable for further investigation
and characterization.
7. Viscosity
The viscosity of the chitosan was determined by using a digital rotary viscosimeter at 20C°
equipped with a spindle in the rotational speed and 20 revolutions per min , Chitosan solution
was prepared in 1% acetic acid at 1% concentration on a dry basis. (NURHAYATI and
TANG, 2022).
8. Molecular wight
According to (ZARGAR et al., 2015) Molecular weight can be determined by Viscometry
which is a fast and uncomplicated route for the determination of the molecular weight.
The constants a and K in the Mark-Houwink equation are determined.
The intrinsic viscosity is expressed as:
5
í µí³¤ = í µí² í µí±´ í µí²
Where :
í µí² = 1.81 × 10
−3
and í µí² = 0.93
➢ Pipettes
Procedure :
➢ 1% Chitosan solution is prepared by dissolving 0.2g of chitosan powder in 20 ml of
purified water
➢ 1% chitin solution is prepared by dissolving 0.2g of chitin powder in 20 ml of purified
water
➢ Protein reference solution is prepared by diluting fresh egg white with same amount of
purified water
➢ Using a pipette, add 1-5 ml of biuret reagent to each tube and mix well
➢ Allow the test tubes to stand for 5-10 minutes at room temperature.
➢ Observe any color change, The appearance of a violet color indicates the presence of
protein in the sample.
6. Solubility
A dissolution experiment was conducted by introducing a predetermined quantity of chitosan
powder into a 1% acetic acid solution. The solution was subjected to stirring for an extended
period of time, specifically overnight, at ambient temperature. (SULARSIH et al., 2012)
The purpose of this prolonged stirring was to facilitate the dissolution process and ensure the
complete integration of the chitosan powder within the acetic acid solution. This experimental
procedure aimed to obtain a homogeneous chitosan solution suitable for further investigation
and characterization.
7. Viscosity
The viscosity of the chitosan was determined by using a digital rotary viscosimeter at 20C°
equipped with a spindle in the rotational speed and 20 revolutions per min , Chitosan solution
was prepared in 1% acetic acid at 1% concentration on a dry basis. (NURHAYATI and
TANG, 2022).
8. Molecular wight
According to (ZARGAR et al., 2015) Molecular weight can be determined by Viscometry
which is a fast and uncomplicated route for the determination of the molecular weight.
The constants a and K in the Mark-Houwink equation are determined.
The intrinsic viscosity is expressed as:
5
í µí³¤ = í µí² í µí±´ í µí²
Where :
í µí² = 1.81 × 10
−3
and í µí² = 0.93