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VI.
Characterization of Chitosan Hydrochloride
All the tests below are conducted according to European Pharmacopoeia 6th Edition 2008.
Character:
Identification :
1. IR spectroscopy
IR spectra of chitosan sample is recorded using IR spectrophotometer in the range of 400–
4000 cm−1, in the Physical Lab, Saidal Group, Algeria
2. S solution preparation
Dissolve 1g of chitosan hydrochloride in 100 ml of water R, maintain under vigorous
mechanical stirring for 20 min
Test 01
Test 02
• take 50 ml of S solution and
complete with 250 ml of 25%
ammonia solution.
✓ a voluminous gelatinous mass is
formed.
• for 10 ml of S solution add 90 ml of
acetone.
✓ a voluminous gelatinous mass is
formed.
3. Test 01 : The a- chloride reaction
Procedure:
➢ Use 2 ml of the prescribed solution S
➢ acidify with diluted nitric acid R
➢ add 0.4 ml of the silver nitrate solution R1
➢ stir and let stand, a white curd precipitate form.
➢ centrifuge and wash 3 times with 1 ml of water, perform this operation quickly away
from bright taking into account the fact that the supernatant does not become perfectly
clear.
➢ suspend the precipitate in 2ml of water and add 1.5 ml of ammonia
➢ the precipitate dissolves easily except for any large particles which dissolve slowly.
4. Ph
✓ 4-6 for S solution
Appearance
Solubility
fine white or almost white powder
fairly soluble in water practically insoluble
in ethanol