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Chitosan from chitin by alkaline hydrolysis:
The N-deacetylation of the demineralized product was carried out by using 55% NaOH, with
weight to volume ratio of 1g:5ml at 100˚C for 12 h. The product was washed with distilled
water and dried overnight.
Method 03
This method was conducted by (AHING AND WID, 2016) with modifications.
Protein removal:
Calcium carbonate removal:
Samples from deproteination process were added with 1.0 M HCl in the ratio 1:16 (w/v) and
allowed to stand for 24 hours, with pH value ranged pH 1.0-2.5 at room temperature. After that,
the solution was filtered and the samples were washed with distilled water until neutral pH was
achieved (pH6.5-8.0). The samples were then dried using an oven at 80C° until constant weight
was obtained. The dried sample is now known as chitin
Figure 30 : Method 02 - alkaline hydrolysis (A) and washing process
Figure 31 : Method 03deproteinization process
A total of 15 g samples of raw shrimp shell waste were added with
2.0 M NaOH in the ratio 1:16 (w/v) then left for 48 hours at room
temperature, with pH ranged from 11-13.
After that, the solution was filtered and the samples were washed
with distilled water until neutral pH was achieved (pH6.5-8.0).
Water from the samples was removed before performing the
demineralization process.
A