35
Chitosan from chitin by alkaline hydrolysis:
The deacetylation process was conducted by soaking dried chitin prepared from
demineralization in a 48% NaOH for 48 hours at room temperature. After two days, the product
is known as chitosan. Chitosan was washed with tap water until neutral (pH6.5-8.0) and dried
as described in deproteination and demineralization. brownish powder was obtained.
The final hybrid method
For a better chitin and chitosan extraction, choosing a method that combines the strengths of
three other previous methods can be a great strategy for achieving optimal results.
The process of developing a hybrid method may involve conducting experiments, analysing
data, until we arrive at a final product that is robust, effective, and well-suited
In this particular approach, a quantity of 100 grams of shrimp shell powder was utilized to
undergo a conversion process.
Procedure:
Table 10: Table of the final Method
Protein removal
3h alkaline treatment
With NaOH 5%
Heat: 60-70 C°
Filtered and dried in oven
80 C°