33
Method 02
This method was conducted according to (KANDILE et al, 2018) with modifications.
Protein removal:
The deproteinization process was carried out by weight 15g of shrimp shells powder by using
5% NaOH with a weight to volume ratio of 1g:8ml (w/v).
The solution with shrimp shells was refluxed at 70˚C for 3 h, The product was collected and
washed until clear solution, it was then dried in a vacuum
The product was decolorized with pure acetone for 24 h, then collected and washed to neutrality,
then dried.
Calcium carbonate removal:
The decolorized product was demineralized by using 1% HCl, with a weight to volume ratio of
1g:10ml for 24 h at room temperature. The product was collected and washed to give light
brown powder.
Figure 28 : Method 02 – bleaching (A) and washing
process (B)
Figure 29 : Method 02 -resulting chitin powder
A
B