Table 4
(continued)
Format
Analytes
Sample
volume
(mL)
Sample
preparation
Antibodies
Competitive
hapten
Cross-reactivity
a
Detection
Recovery
(urine, %)
IC
50
(ng/mL)
Sensitivity
(ng/mL)
Ref.
Quenching
fluoroimmunoassay
3PBAG
0.002
Dilution
(1,000-fold)
Primary: pAb
(rabbit)
F3PBAG
3PBAlc 6.2%, esfenvalerate
1.4%, 3PBA 0.35%, N-[(S)4-chloro-α-(1-methylethyl)
benzene-acetyl]glycine
<0.02%, permethrin
<0.02%
Fluorometric
(490/512 nm)
85–111
1.2
(buffer)
DL: 0.25
(buffer)
[50]
Indirect competitive ELISA
3PBAG
10
SPE (C18),
elution with
methanol
Primary: pAb
(rabbit)
Secondary:
GAR-HRP
3PBA-BSA
Fluvalinate 0.07%,
esfenvalerate 0.04%, methyl
3-phenoxybenzoate 0.03%,
3PBA 0.02%, 3PBAlc
0.01%, benzamidoacetic
acid
<0.01%, sFAG
<0.01%, CPBA
<0.01%,
(4-chlorophenyl)-3methylbutanoate
<0.01%,
{[(2R)-2-(4-chlorophenyl)3-methylbutanoyl]amino}
acetic acid
<0.01%, DCCA
dimethylcyclopropane1-carboxylate
<0.01%,
cypermethrin
<0.01%
UV-VIS
(450–650 nm)
98–109
0.42
(buffer)
LOQ: 1
(urine)
[43]
sFAG
Primary: pAb
(rabbit)
Secondary:
GAR-HRP
Hapten 3
e
-
BSA
{[(2R)-2-(4-chlorophenyl)3-methylbutanoyl]amino}
acetic acid 2.8%, methyl
(2S)-2-(4-chlorophenyl)-3methylbutanoate 0.3%,
(2S)-2-(4-chlorophenyl)3-methylbutanoic acid
0.08%,
fluvalinate 0.02%,
esfenvalerate 0.02%,
3PBAG 0.01%, methyl 3phenoxybenzoate
<0.01%,
3PBA
<0.01%, 3PBAlc
95–115
0.40
(buffer)
74
B. Wielgomas et al.