DCCA
<0.06%, transDCCA
<0.06%, transDCCA-glycine
<0.06%,
cypermethrin
<0.06%, permethrin
<0.06%,
esfenvalerate
<0.06%,
cyfluthrin
<0.06%
Noncompetitive
magnetic beadbased PHAIA
3PBA
0.5
Direct dilution with
buffer (25fold) or
mixed-mode
SPE
(C8 + SAX)
eluted with
1% acetic
acid in 70:30
Hex/EtAc
Anti-analyte:
protein Apurified pAb
(immobilized
on magnetic
beads)
Anti-phage:
MAb-HRP
NA
NR
UV-VIS
(450 nm)
87–109
SC
50 :
0.2–0.4
NR
[48]
Noncompetitive
PHAIA real-time
PCR
3PBA
0.05
Dilution
Anti-analyte:
protein Apurified pAb
Anti-phage:
MAb-HRP
NA
NR
Fluorometric
80–115
NA
LOD: 0.02
(buffer)
[53]
Indirect competitive VELISA
3PBA
0.002
Dilution with
buffer (25fold)
Primary: soluble VHH
(alpaca)
Secondary:
goat anti-HA
tag PAb-HRP
3PBA-BSA
4OH3PBA 150%, 3phenoxybenzaldehyde 10%
d
UV-VIS
(450–650 nm)
80–110
1.4
(buffer)
LOD: 0.1
(buffer)
[42]
Indirect competitive PELISA
Primary:
phagedisplayed
VHH
Secondary:
goat anti-M13
phage MAbHRP
NR
0.1
(buffer)
LOD:
0.01
(buffer)
(continued)
Analytical Methods for Determination Urinary Metabolites of Synthetic. . .
73