hyperbranched PEI [89]. Uekama et al. constructed a series of hyperbranched CD
polymers using polyaminomethylene and CDs with gene delivery capabilities (Fig.
12) [90–95]. They firstly synthesized hyperbranched polyamidoamine dendrimer
with α-, β-, and γ-CDs, respectively [90]. These hyperbranched CD polymers can
condense pDNA and protect pDNA from being degraded by DNase I. Compared
with those hyperbranched polyamidoamine dendrimers without CDs or the mixture
of hyperbranched polyamidoamine dendrimers and CDs, these hyperbranched CD
polymers showed an effectively luciferase gene expression, especially in the hyperbranched α-CD polymers which presented the highest transfection activity. Furthermore, the hyperbranched α-CD polymers have the superior gene transfer activity
than Lipofectin reagent (commercial cell transfection reagent). Then they prepared
Fig. 12 Chemical structures of a series of hyperbranched CD polymers using polyaminomethylene
and CDs constructed by Uekama [90–92]. (Reprinted (adapted) with permission from Ref. [90].
Copyright (2001) American Chemical Society.) (Reprinted (adapted) with permission from Ref.
[91]. Copyright (2002) American Chemical Society.) (Reprinted (adapted) with permission from
Ref. [92]. Copyright (2003) American Chemical Society)
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