481
Table 3 Morphological and biochemical characteristics of native rhizobia isolates nodulating cowpea
Isolate characteristics
Group
Size
(mm)
Elevation Margin
BTB
reaction
EPS
production
Colour
Congo red
reaction
YEMA
reaction
Texture Colony Growth Transparency
i
1.25
F
sm
B
Dry
w
crna
non
Mfg
shinny cnt
o
ii
6.8
D
sm
Y
M
cr
crna + pp
non
Mfg
shinny cnt
t
iii
2
D
sm
Y
Dry
crw
cna
non
Mfg
shinny cnt
t
iv
4.25
D
sm
Y
M
cr
crna + pp
non
Mfg
creamy cnt
t
v
3.5
D
sm
Y
M
crw + opc crna + pk
non
Mfg
shinny cnt
t
vi
3.8
D
sm
Y
M
crw + opc crna
non
mfg
shinny cnt
t
vii
6.1
Cnx
sm + irr Y
M
crw + opc crna + pp
non
mfg
shinny cnt
t
viii
3.2
Cnx
sm
Y
M
cr
crna
non
mfg
shinny cnt
t
ix
3.25
Cnx
sm
Y
M
crw + opc crna + pp
non
mfg
shinny cnt
t
x
1
f
sm
B
dry
w
crna
non
mfg
shinny cnt
t
xi
3.7
d
sm
Y
m
w
crna + pk
non
mfg
shinny cnt
tp
xii
4.5
cnx
sm
Y
m
cr
crna + pp
non
mfg
shinny cnt
t
xiii
3.6
d
sm
Y
m
w
crna + pk
non
mfg
shinny cnt
t
xiv
6
cnx
sm
Y
m
w
crna + pp
non
mfg
shinny cnt
t
xv
3.6
Cnx
sm
Y
m
cr
crna + pp
non
mfg
shinny cnt
t
xvi
7
d
sm
Y
C
cr
crna
non
mfg
shinny cnt
t
xvii
3.5
d
sm
B
m
w
crna + pk
non
mfg
shinny cnt
t
xviii 3.25
d
sm
B
m
w
crna + pk
non
mfg
shinny cnt
t
xix
2.8
d
sm
Y
m
cr
crna
non
mfg
shinny cnt
t
xx
2.9
Cnx
sm
Y
m
w
crna + pp
non
mfg
shinny cnt
t
xxi
3.2
d
sm + irr Y
m
w
crna + pk
non
mfg
shinny cnt
t
xxii
4.4
Cnx
sm
B
m
w
crna + pk
non
mfg
shinny cnt
t
f
flat, d
domed, cnx
convex, sm
smooth, sm + irr
smooth irregular, B
blue, Y
yellow, M
moderate, w
white, cr
creamy, crw + opc
creamy white with opaque
centre, crna
Congo red non-absorbing, crna + pp
Congo red non-absorbing with purple pigmentation on media, crna + pk
Congo red non-absorbing with pink
colour appearance, mfg
mucoid firm gummy, cnt
confluent, t
translucent, tp
transparent, o
opaque
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