209
5. 10 mM 4-(2-Hydroxyethyl)-1-piperazine-ethanesulfonic acid.
6. 2 mM L-glutamine.
7. 100 U/ml penicillin.
8. 100 U/ml streptomycin.
9. 1 N HCL.
10. 1 N NAOH.
11. 3.7 g/l Sodium bicarbonate (49.3 mL) per liter of DMEM.
1. Cellulose membrane dialysis tube.
2. Receptor solution (sodium acetate buffer pH 5.5).
1. Dissolve 500 mg MTT powder in 10 mL PBS.
2. Stir the mixture with a magnetic stirrer for approximately 1 h
in the dark.
3. Filter sterilize solution with a 0.22 mm Millipore filter.
4. Store in 10 mL aliquots at −20 °C.
1. Plate shaker.
2. Pipettes, 0.001–1 mL single channel and 0.01–0.3
multichannel.
3. Class 2B safety cabinet.
4. Benchtop centrifuge.
5. Microplate reader (ELISA reader).
6. Incubator with 5% O 2 suitable for drug experiments.
7. Microplate 96 well.
8. Circulating hot water bath.
9. UV/VIS spectrophotometer.
10. Graduated glass container.
11. Ultrasonic homogenizers supplied with pulsed horn.
12. pH meter.
13. Glassware.
14. Refrigerator.
15. 15 Hot-plate stirrer.
1. Assess the statistical significance of differences between groups
by two-tailed Student’s t-test.
2. Present the data as means of ±S.D. of four independent experiments. Values with p < 0.05 were considered as significant.
2.3 In Vitro Release
Assessment
2.4 Preparation MTT
Assay Stock Solution
2.5 Equipment
and Instruments
2.6 Statistical
Analysis
Antitumor Efficacy of Ceranib-2 with Nano-Formulation of PEG and Rosin Esters
5. 10 mM 4-(2-Hydroxyethyl)-1-piperazine-ethanesulfonic acid.
6. 2 mM L-glutamine.
7. 100 U/ml penicillin.
8. 100 U/ml streptomycin.
9. 1 N HCL.
10. 1 N NAOH.
11. 3.7 g/l Sodium bicarbonate (49.3 mL) per liter of DMEM.
1. Cellulose membrane dialysis tube.
2. Receptor solution (sodium acetate buffer pH 5.5).
1. Dissolve 500 mg MTT powder in 10 mL PBS.
2. Stir the mixture with a magnetic stirrer for approximately 1 h
in the dark.
3. Filter sterilize solution with a 0.22 mm Millipore filter.
4. Store in 10 mL aliquots at −20 °C.
1. Plate shaker.
2. Pipettes, 0.001–1 mL single channel and 0.01–0.3
multichannel.
3. Class 2B safety cabinet.
4. Benchtop centrifuge.
5. Microplate reader (ELISA reader).
6. Incubator with 5% O 2 suitable for drug experiments.
7. Microplate 96 well.
8. Circulating hot water bath.
9. UV/VIS spectrophotometer.
10. Graduated glass container.
11. Ultrasonic homogenizers supplied with pulsed horn.
12. pH meter.
13. Glassware.
14. Refrigerator.
15. 15 Hot-plate stirrer.
1. Assess the statistical significance of differences between groups
by two-tailed Student’s t-test.
2. Present the data as means of ±S.D. of four independent experiments. Values with p < 0.05 were considered as significant.
2.3 In Vitro Release
Assessment
2.4 Preparation MTT
Assay Stock Solution
2.5 Equipment
and Instruments
2.6 Statistical
Analysis
Antitumor Efficacy of Ceranib-2 with Nano-Formulation of PEG and Rosin Esters