210
3 Methods
1. In 50 mL glass beaker, dissolve 1 mg of ceranib-2, and 1 mg
of rosin ester powder in 0.1 mL of dimethyl sulfoxide (DMOS).
2. Place the mixture in 50 mL beaker.
3. Make up to 50 mL with ethyl acetate till fill line.
4. Mix the mixture using a stirrer at 1500 rpm for 10 min at
room temperature, before the addition of an appropriate volume of ethyl acetate till fill line.
1. Dissolve 0.5 g of polyethylene glycol (PEG 400) in 77.6 mL
of deionized water in a 50 mL glass beaker at room temperature using a stirrer.
2. Make up to 50 mL with ethyl acetate till fill line.
3. Store at room temperature.
1. Place the contentious-phase beaker under sonication transducer horn (see Note 1).
2. Turn power on the ultrasonic generator device (see Note 2).
3. Set up sonication-independent parameter variables such as
amplitude and time, here samples 20 kHz, for 20 min, at room
temperature (see Note 3).
4. Dropwise and using a Pasteur pipette add the previously prepared dispersion-phase mixture into the continuous phase
while sonicating the mixture for 20 min (see Note 4).
5. Remove excess ethyl acetate residues from the emulsion by
leaving the sample to evaporate for 6 h at 40 °C using hot
plate (Fig. 5).
6. Store samples at −4 °C until submitted for freeze-drying or
for further characterization.
1. Prepare 800 mL of distilled water in 1 L glass container.
2. Using laboratory balance, weight 7.719 g sodium acetate and
add it to the 800 mL of water prepared previously.
3. Add 0.353 g of acetic acid to the solution.
4. Using pH meter adjust pH using HCL or NAOH.
5. Make up the volume to 1 L with distilled water.
1. Using scissors, cut appropriate length of cellulose membrane
dialysis tube suitable for 5 mL of sample.
2. Place a 2 mL of PREC-2 NPs into the membrane using pipette,
and close it tightly from both ends (Fig. 6).
3.1 Preparation
of Dispersion Phase
3.2 Preparation
of Continuous Phase
3.3 Synthesis
of PREC-2 NPs
3.4 Preparation
of Physiological
Reception Solution
for In Vitro Release
Experiment
3.5 In Vitro DrugRelease Profile
of PREC-2 NPs
Ali Ben Taleb et al.
3 Methods
1. In 50 mL glass beaker, dissolve 1 mg of ceranib-2, and 1 mg
of rosin ester powder in 0.1 mL of dimethyl sulfoxide (DMOS).
2. Place the mixture in 50 mL beaker.
3. Make up to 50 mL with ethyl acetate till fill line.
4. Mix the mixture using a stirrer at 1500 rpm for 10 min at
room temperature, before the addition of an appropriate volume of ethyl acetate till fill line.
1. Dissolve 0.5 g of polyethylene glycol (PEG 400) in 77.6 mL
of deionized water in a 50 mL glass beaker at room temperature using a stirrer.
2. Make up to 50 mL with ethyl acetate till fill line.
3. Store at room temperature.
1. Place the contentious-phase beaker under sonication transducer horn (see Note 1).
2. Turn power on the ultrasonic generator device (see Note 2).
3. Set up sonication-independent parameter variables such as
amplitude and time, here samples 20 kHz, for 20 min, at room
temperature (see Note 3).
4. Dropwise and using a Pasteur pipette add the previously prepared dispersion-phase mixture into the continuous phase
while sonicating the mixture for 20 min (see Note 4).
5. Remove excess ethyl acetate residues from the emulsion by
leaving the sample to evaporate for 6 h at 40 °C using hot
plate (Fig. 5).
6. Store samples at −4 °C until submitted for freeze-drying or
for further characterization.
1. Prepare 800 mL of distilled water in 1 L glass container.
2. Using laboratory balance, weight 7.719 g sodium acetate and
add it to the 800 mL of water prepared previously.
3. Add 0.353 g of acetic acid to the solution.
4. Using pH meter adjust pH using HCL or NAOH.
5. Make up the volume to 1 L with distilled water.
1. Using scissors, cut appropriate length of cellulose membrane
dialysis tube suitable for 5 mL of sample.
2. Place a 2 mL of PREC-2 NPs into the membrane using pipette,
and close it tightly from both ends (Fig. 6).
3.1 Preparation
of Dispersion Phase
3.2 Preparation
of Continuous Phase
3.3 Synthesis
of PREC-2 NPs
3.4 Preparation
of Physiological
Reception Solution
for In Vitro Release
Experiment
3.5 In Vitro DrugRelease Profile
of PREC-2 NPs
Ali Ben Taleb et al.