48
involved in these studies. With growing interest in deciphering the fate and behaviour of microorganisms in the environment and the increased number of activities
on a plethora of methodological expertise, it has now become most demanding and
challenging to extract accurate and reproducible knowledge from the metagenomic
sequence information and link this knowledge to functions.
4.2.4 Metagenomics: Success Stories
Among the enormous applications of metagenomics, the most important ones
include the exploration of environmental samples, the gut microbiome, unidentified
microbes, genetic elements, underlying interlinked processes, cleanup of sewage
and solid waste, and the implications of the huge amounts of meta-sequence data
coming from the unseen microbial communities (Handelsman 2004; Bashir et al.
2014; Sebastian et al. 2013; Wang et al. 2016). Metagenomics in combination with
the metatranscriptome and proteome can provide better insight into the soil
Fig. 4.5 Projection of a particular metagenomic dataset onto KEGG pathways through
KeggMapper tool of MG-RAST server (dark blue lines represent query metagenomic dataset
mapped over different pathways)
4 Single-Cell Genomics and Metagenomics for Microbial Diversity Analysis
involved in these studies. With growing interest in deciphering the fate and behaviour of microorganisms in the environment and the increased number of activities
on a plethora of methodological expertise, it has now become most demanding and
challenging to extract accurate and reproducible knowledge from the metagenomic
sequence information and link this knowledge to functions.
4.2.4 Metagenomics: Success Stories
Among the enormous applications of metagenomics, the most important ones
include the exploration of environmental samples, the gut microbiome, unidentified
microbes, genetic elements, underlying interlinked processes, cleanup of sewage
and solid waste, and the implications of the huge amounts of meta-sequence data
coming from the unseen microbial communities (Handelsman 2004; Bashir et al.
2014; Sebastian et al. 2013; Wang et al. 2016). Metagenomics in combination with
the metatranscriptome and proteome can provide better insight into the soil
Fig. 4.5 Projection of a particular metagenomic dataset onto KEGG pathways through
KeggMapper tool of MG-RAST server (dark blue lines represent query metagenomic dataset
mapped over different pathways)
4 Single-Cell Genomics and Metagenomics for Microbial Diversity Analysis