12 Industrial Extraction of Microalgal Pigments
297
Table 12.5
(continued)
Study
Species
Pigment
Biomass state
Cell rupture and extraction
conditions
Maximum
yield/recovery at
optimum conditions
Key findings
Gantar et al.
(2012)
Limnotrhix
sp C-phycocyanin
(C-PC)
Dewatered
concentrate
Repeated (3x) freeze-thawing
in distilled water. The lysate
was filtered and subjected to a
sequence of purification steps:
mixing with activated carbon
(1% w/v) and chitosan
(0.01 g/l), ammonium sulphate
precipitation (20–60%) and
concentration with tangential
flow filtration (30 kDa)
180 mg C-PC/g
biomass with a purity
ratio (A
620 /A
680 ) of
2.0 without
purification, 80 mg
C-PC/g biomass with
a purity ratio 4.3 with
the use of all 3
purification steps
Subjecting C-PC
extract to purification
steps increased its
purity but reduced its
overall yield or
recovery
297
Table 12.5
(continued)
Study
Species
Pigment
Biomass state
Cell rupture and extraction
conditions
Maximum
yield/recovery at
optimum conditions
Key findings
Gantar et al.
(2012)
Limnotrhix
sp C-phycocyanin
(C-PC)
Dewatered
concentrate
Repeated (3x) freeze-thawing
in distilled water. The lysate
was filtered and subjected to a
sequence of purification steps:
mixing with activated carbon
(1% w/v) and chitosan
(0.01 g/l), ammonium sulphate
precipitation (20–60%) and
concentration with tangential
flow filtration (30 kDa)
180 mg C-PC/g
biomass with a purity
ratio (A
620 /A
680 ) of
2.0 without
purification, 80 mg
C-PC/g biomass with
a purity ratio 4.3 with
the use of all 3
purification steps
Subjecting C-PC
extract to purification
steps increased its
purity but reduced its
overall yield or
recovery