296
R. Halim
Table 12.5
(continued)
Study
Species
Pigment
Biomass state
Cell rupture and extraction
conditions
Maximum
yield/recovery at
optimum conditions
Key findings
Jubeau et al.
(2013)
Porphyridium
cruentum
B-phycoerythrin
(B-PE)
direct culture
from cultivationn
(exponential
phase)
2-stage high-pressure
homogenisation (HPH):
low-pressure HPH
(27–70 MPa) in culture
medium to extract protein,
centriugation, resuspension in
distilled water, then
high-pressure HPH at
270 MPa to recover B-PE
almost 100 wt% of
available B-PE
(3.6 mg B-PE/g
biomass) with a
purity ratio of 0.79
Intracellular proteins
are extracted more
effectively extracted at
low HPH pressure than
B-PE. Hence, with a
two-step HPH
procedure, it is
possible to selectively
remove protein in the
first low-pressure HPH
step and then recover
enriched B-PE with the
second high-pressure
HPH step
Sorensen
et al. (2013)
Galdieria
sulphuraria
C-phycocyanin
(C-PC)
Centrifuged
concentrate
Ball milling followed by
phosphate buffer extraction:
three cycles in 50 mmol/L
potassium phospate buffer (pH
7.2) followed by cell rupture
in a ball mill (3,500 rpm). The
supernatant (or crude extract)
was collected and purified
25–30 mg C-PC/g
biomass
Without further
purification steps, the
crude extract had a low
purity
(continued)