12 Visualizing Mouse Embryo Gastrulation Epithelial-Mesenchymal
Transition Through Single Cell Labeling Followed by Ex Vivo
Whole Embryo Live Imaging. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
Wallis Nahaboo, Bechara Saykali, Navrita Mathiah,
and Isabelle Migeotte
13 Fluorescence Recovery After Photobleaching to Study the Dynamics
of Membrane-Bound Proteins In Vivo Using the Drosophila Embryo . . . . . . . . . 145
Joshua Greig and Natalia A. Bulgakova
14 Methods to Generate and Assay for Distinct Stages of Cancer
Metastasis in Adult Drosophila melanogaster . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 161
Jamie Adams, Andreu Casali, and Kyra Campbell
PART IV IN VIVO IMAGING OF MET
15 4D Live Imaging and Analysis of Chick Embryo Somites . . . . . . . . . . . . . . . . . . . . 173
Gi Fay Mok, James McColl, and Andrea Mu ¨nsterberg
16 In Vivo Analysis of the Mesenchymal-to-Epithelial Transition
During Chick Secondary Neurulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 183
Elena Gonzalez-Gobartt, Guillaume Allio, Bertrand Be ´naze ´raf, and Elisa
Martı ´
17 Multiscale In Vivo Imaging of Collective Cell Migration
in Drosophila Embryos. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 199
Gordana Scepanovic, Alexandru Florea, and Rodrigo Fernandez-Gonzalez
PART V EX VIVO/IN VITRO METHODS TO STUDY EMT/MET
18 Methods to Generate Tube Micropatterns for Epithelial
Morphogenetic Analyses and Tissue Engineering . . . . . . . . . . . . . . . . . . . . . . . . . . . 227
Minerva Bosch-Fortea and Fernando Martı ´n-Belmonte
19 CAFs and Cancer Cells Co-Migration in 3D Spheroid Invasion Assay . . . . . . . . . 243
Sefora Conti, Takuya Kato, Danielle Park, Erik Sahai, Xavier Trepat,
and Anna Labernadie
20 Using Xenopus Neural Crest Explants to Study Epithelial-Mesenchymal
Transition. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 257
Nade `ge Gouignard, Christian Rouvie `re, and Eric Theveneau
21 Xenopus Deep Cell Aggregates: A 3D Tissue Model
for Mesenchymal-to-Epithelial Transition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 275
Hye Young Kim and Lance A. Davidson
22 Molecular Tension Microscopy of E-Cadherin During
Epithelial-Mesenchymal Transition. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 289
Helena Canever, Pietro Salvatore Carollo, Romain Fleurisson,
Philippe P. Girard, and Nicolas Borghi
viii
Contents
Transition Through Single Cell Labeling Followed by Ex Vivo
Whole Embryo Live Imaging. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
Wallis Nahaboo, Bechara Saykali, Navrita Mathiah,
and Isabelle Migeotte
13 Fluorescence Recovery After Photobleaching to Study the Dynamics
of Membrane-Bound Proteins In Vivo Using the Drosophila Embryo . . . . . . . . . 145
Joshua Greig and Natalia A. Bulgakova
14 Methods to Generate and Assay for Distinct Stages of Cancer
Metastasis in Adult Drosophila melanogaster . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 161
Jamie Adams, Andreu Casali, and Kyra Campbell
PART IV IN VIVO IMAGING OF MET
15 4D Live Imaging and Analysis of Chick Embryo Somites . . . . . . . . . . . . . . . . . . . . 173
Gi Fay Mok, James McColl, and Andrea Mu ¨nsterberg
16 In Vivo Analysis of the Mesenchymal-to-Epithelial Transition
During Chick Secondary Neurulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 183
Elena Gonzalez-Gobartt, Guillaume Allio, Bertrand Be ´naze ´raf, and Elisa
Martı ´
17 Multiscale In Vivo Imaging of Collective Cell Migration
in Drosophila Embryos. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 199
Gordana Scepanovic, Alexandru Florea, and Rodrigo Fernandez-Gonzalez
PART V EX VIVO/IN VITRO METHODS TO STUDY EMT/MET
18 Methods to Generate Tube Micropatterns for Epithelial
Morphogenetic Analyses and Tissue Engineering . . . . . . . . . . . . . . . . . . . . . . . . . . . 227
Minerva Bosch-Fortea and Fernando Martı ´n-Belmonte
19 CAFs and Cancer Cells Co-Migration in 3D Spheroid Invasion Assay . . . . . . . . . 243
Sefora Conti, Takuya Kato, Danielle Park, Erik Sahai, Xavier Trepat,
and Anna Labernadie
20 Using Xenopus Neural Crest Explants to Study Epithelial-Mesenchymal
Transition. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 257
Nade `ge Gouignard, Christian Rouvie `re, and Eric Theveneau
21 Xenopus Deep Cell Aggregates: A 3D Tissue Model
for Mesenchymal-to-Epithelial Transition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 275
Hye Young Kim and Lance A. Davidson
22 Molecular Tension Microscopy of E-Cadherin During
Epithelial-Mesenchymal Transition. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 289
Helena Canever, Pietro Salvatore Carollo, Romain Fleurisson,
Philippe P. Girard, and Nicolas Borghi
viii
Contents