PART VI METHODS TO STUDY EPITHELIAL-MESENCHYMAL
PLASTICITY AT THE SINGLE CELL, SUBCELLULAR,
AND MOLECULAR LEVEL
23 Methodologies for Following EMT In Vivo at Single Cell Resolution . . . . . . . . . 303
Abdull J. Massri, Geoffrey R. Schiebinger, Alejandro Berrio,
Lingyu Wang, Gregory A. Wray, and David R. McClay
24 Isolation and Identification of EMT Subtypes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 315
Robert J. Norgard and Ben Z. Stanger
25 Studying the Metabolism of Epithelial-Mesenchymal Plasticity
Using the Seahorse XFe96 Extracellular Flux Analyzer . . . . . . . . . . . . . . . . . . . . . . 327
Sugandha Bhatia, Erik W. Thompson, and Jennifer H. Gunter
26 Inducing Sequential Cycles of Epithelial-Mesenchymal
and Mesenchymal-Epithelial Transitions in Mammary Epithelial Cells . . . . . . . . . 341
Cecile Davaine, Eva Hadadi, William Taylor,
Annelise Bennaceur-Griscelli, and Herve ´ Acloque
27 Analysis of Cellular EMT States Using Molecular Biology
and High Resolution FISH Labeling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 353
Noe ´mie Kempf, Fatima Moutahir, Isabelle Goiffon, Sylvain Cantaloube,
Kerstin Bystricky, and Anne-Claire Lavigne
28 Mathematical Modeling of Plasticity and Heterogeneity in EMT . . . . . . . . . . . . . 385
Shubham Tripathi, Jianhua Xing, Herbert Levine,
and Mohit Kumar Jolly
29 Control of Cell Migration Using Optogenetics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 415
Leo Valon and Simon de Beco
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 427
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