Contents
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
v
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
xi
PART I PERSPECTIVES ON THE PAST AND FUTURE OF EMT RESEARCH
1 A Spectrum of Cell States During the Epithelial-to-Mesenchymal
Transition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3
Erica J. Hutchins and Marianne E. Bronner
2 Perspective on Epithelial-Mesenchymal Transitions in Embryos. . . . . . . . . . . . . . .
7
David R. McClay
3 EMT, One of Many Morphological Transitions in Cellular Phase Space. . . . . . . . 13
Denise J. Montell
4 Are You Interested or Afraid of Working on EMT?. . . . . . . . . . . . . . . . . . . . . . . . . . 19
M. Angela Nieto
5 Partial EMT/MET: An Army of One . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Sofiane Hamidi, Hiroki Nagai, and Guojun Sheng
6 EMT: An Update . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Jean Paul Thiery
PART II REVIEW ON MESENCHYMAL-TO-EPITHELIAL TRANSITIONS
7 Mesenchymal-to-Epithelial Transitions in Development and Cancer. . . . . . . . . . . 43
John-Poul Ng-Blichfeldt and Katja Ro ¨per
PART III METHODS TO STUDY EMT IN IN VIVO SYSTEMS
8 Live Imaging of Epithelial-Mesenchymal Transition in Mesoderm
Cells of Gastrulating Drosophila Embryos . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Lingkun Gu and Mo Weng
9 Zebrafish Neural Crest: Lessons and Tools to Study In Vivo
Cell Migration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Zain Alhashem, Macarena Alvarez-Garcillan Portillo,
Mint Ravinand Htun, Anton Gauert, Luis Briones Montecinos,
Steffen H€ artel, and Claudia Linker
10 Live Imaging of the Neural Crest Cell Epithelial-to-Mesenchymal
Transition in the Chick Embryo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
Mary Cathleen McKinney and Paul M. Kulesa
11 Exploiting Drosophila melanogaster Wing Imaginal Disc Eversion
to Screen for New EMT Effectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
Sofia Golenkina, Rosemary Manhire-Heath, and Michael J. Murray
vii
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
v
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
xi
PART I PERSPECTIVES ON THE PAST AND FUTURE OF EMT RESEARCH
1 A Spectrum of Cell States During the Epithelial-to-Mesenchymal
Transition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3
Erica J. Hutchins and Marianne E. Bronner
2 Perspective on Epithelial-Mesenchymal Transitions in Embryos. . . . . . . . . . . . . . .
7
David R. McClay
3 EMT, One of Many Morphological Transitions in Cellular Phase Space. . . . . . . . 13
Denise J. Montell
4 Are You Interested or Afraid of Working on EMT?. . . . . . . . . . . . . . . . . . . . . . . . . . 19
M. Angela Nieto
5 Partial EMT/MET: An Army of One . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Sofiane Hamidi, Hiroki Nagai, and Guojun Sheng
6 EMT: An Update . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Jean Paul Thiery
PART II REVIEW ON MESENCHYMAL-TO-EPITHELIAL TRANSITIONS
7 Mesenchymal-to-Epithelial Transitions in Development and Cancer. . . . . . . . . . . 43
John-Poul Ng-Blichfeldt and Katja Ro ¨per
PART III METHODS TO STUDY EMT IN IN VIVO SYSTEMS
8 Live Imaging of Epithelial-Mesenchymal Transition in Mesoderm
Cells of Gastrulating Drosophila Embryos . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Lingkun Gu and Mo Weng
9 Zebrafish Neural Crest: Lessons and Tools to Study In Vivo
Cell Migration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Zain Alhashem, Macarena Alvarez-Garcillan Portillo,
Mint Ravinand Htun, Anton Gauert, Luis Briones Montecinos,
Steffen H€ artel, and Claudia Linker
10 Live Imaging of the Neural Crest Cell Epithelial-to-Mesenchymal
Transition in the Chick Embryo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
Mary Cathleen McKinney and Paul M. Kulesa
11 Exploiting Drosophila melanogaster Wing Imaginal Disc Eversion
to Screen for New EMT Effectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
Sofia Golenkina, Rosemary Manhire-Heath, and Michael J. Murray
vii