RhoGTPases in leading and following cells.
Nat Cell Biol 9:1392–1400
4. Martin P, Lewis J (1992) Actin cables and epidermal movement in embryonic wound healing. Nature 360:179–183. https://doi.org/
10.1038/360179a0
5. McCluskey J, Hopkinson-Woolley J, Luke B,
Martin P (1993) A study of wound healing in
the E11.5 mouse embryo by light and electron
microscopy. Tissue Cell 25:173–181. https://
doi.org/10.1016/0040-8166(93)90017-F
6. Davidson LA, Ezin AM, Keller R (2002)
Embryonic wound healing by apical contraction and ingression in Xenopus laevis. Cell
Motil
53:163–176.
https://doi.org/10.
1002/cm.10070
7. Clark AG, Miller AL, Vaughan E, Yu H-YE,
Penkert R, Bement WM (2009) Integration
of single and multicellular wound responses.
Curr Biol 19:1389–1395. https://doi.org/
10.1016/j.cub.2009.06.044
8. Hunter MV, Willoughby PM, Bruce AEE,
Fernandez-Gonzalez R (2018) Developmental
cell oxidative stress orchestrates cell polarity to
promote embryonic wound healing. Dev Cell
47:377–387.e374
9. Ciruna B, Rossant J (2001) FGF signaling regulates mesoderm cell fate specification and
morphogenetic movement at the primitive
streak. Dev Cell 1(1):37–49
10. Tikhmyanova N, Golemis EA (2011) NEDD9
and BCAR1 negatively regulate E-cadherin
membrane
localization,
and
promote
E-cadherin degradation. PLoS One 6(7):
e22102. https://doi.org/10.1371/journal.
pone.0022102
11. Carvalho L, Jacinto A, Matova N (2014) The
Toll/NF-kappaB signaling pathway is required
for epidermal wound repair in Drosophila. Proc
Natl Acad Sci U S A 111:E5373–E5382.
https://doi.org/10.1073/pnas.1408224111
12. Hunter MV, Lee DM, Harris TJC, Gonzalez
RF (2015) Polarized E-cadherin endocytosis
directs actomyosin remodeling during embryonic wound repair. J Cell Biol 210:801–816.
https://doi.org/10.1083/jcb.201501076
13. Matsubayashi Y, Gilmer CC, Millard TH
(2015) Endocytosis-dependent coordination
of multiple actin regulators is required for
wound healing. J Cell Biol 210:5–7
14. Campbell K, Casanova J (2016) A common
framework for EMT and collective cell migration. Development 143:4291–4300. https://
doi.org/10.1242/dev.139071
15. Wood W, Jacinto A, Grose R, Woolner S,
Gale J, Wilson C, Martin P (2002) Wound
healing recapitulates morphogenesis in
Drosophila embryos. Nat Cell Biol 4:1–7.
https://doi.org/10.1038/ncb875
16. Abreu-Blanco MT, Verboon JM, Liu R, Watts
JJ, Parkhurst SM (2012) Drosophila embryos
close epithelial wounds using a combination of
cellular protrusions and an actomyosin purse
string. J Cell Sci 125:5984–5997. https://doi.
org/10.1242/jcs.109066
17. Hidalgo-Carcedo C, Hooper S, Chaudhry SI,
Williamson P, Harrington K, Leitinger B, Sahai
E (2011) Collective cell migration requires
suppression of actomyosin at cell-cell contacts
mediated by DDR1 and the cell polarity regulators Par3 and Par6. Nat Cell Biol 13
(1):49–58.
https://doi.org/10.1038/
ncb2133
18. Brock J, Midwinter K, Lewis J, Martin P
(1996) Healing of incisional wounds in the
embryonic chick wing bud: characterization of
the actin purse-string and demonstration of a
requirement for rho activation. J Cell Biol
135:1097–1107
19. Ducuing A, Vincent S (2016) The actin cable is
dispensable in directing dorsal closure dynamics but neutralizes mechanical stress to prevent
scarring in the Drosophila embryo. Nat Cell
Biol 18:1149–1160. https://doi.org/10.
1038/ncb3421
20. Zulueta-Coarasa T, Fernandez-Gonzalez R
(2018) Dynamic force patterns promote collective cell movements during embryonic
wound repair. Nat Phys 14:750–758. https://
doi.org/10.1038/s41567-018-0111-2
21. Oreopoulos J, Berman R, Browne M (2014)
Spinning-disk confocal microscopy: present
technology and future trends. In: Quantitative
imaging in cell biology, vol 123, 1st edn. Elsevier Inc., Amsterdam, pp 153–175. https://
doi.org/10.1016/B978-0-12-420138-5.
00009-4
22. Huisken J, Swoger J, Del Bene F, Wittbrodt J,
Stelzer EH (2004) Optical sectioning deep
inside live embryos by selective plane illumination
microscopy.
Science
305
(5686):1007–1009.
https://doi.org/10.
1126/science.1100035
23. Schmied C, Tomancak P (2016) Sample preparation and mounting of Drosophila embryos
for multiview light sheet microscopy. In: Drosophila: methods and protocols. Springer
New York, New York. https://doi.org/10.
1007/978-1-4939-6371-3_10
24. Gustafsson N, Culley S, Ashdown G, Owen
DM, Pereira PM, Henriques R (2016) Fast
live-cell conventional fluorophore nanoscopy
with ImageJ through super-resolution radial
fluctuations. Nat Commun 7:12471
222
Gordana Scepanovic et al.
Nat Cell Biol 9:1392–1400
4. Martin P, Lewis J (1992) Actin cables and epidermal movement in embryonic wound healing. Nature 360:179–183. https://doi.org/
10.1038/360179a0
5. McCluskey J, Hopkinson-Woolley J, Luke B,
Martin P (1993) A study of wound healing in
the E11.5 mouse embryo by light and electron
microscopy. Tissue Cell 25:173–181. https://
doi.org/10.1016/0040-8166(93)90017-F
6. Davidson LA, Ezin AM, Keller R (2002)
Embryonic wound healing by apical contraction and ingression in Xenopus laevis. Cell
Motil
53:163–176.
https://doi.org/10.
1002/cm.10070
7. Clark AG, Miller AL, Vaughan E, Yu H-YE,
Penkert R, Bement WM (2009) Integration
of single and multicellular wound responses.
Curr Biol 19:1389–1395. https://doi.org/
10.1016/j.cub.2009.06.044
8. Hunter MV, Willoughby PM, Bruce AEE,
Fernandez-Gonzalez R (2018) Developmental
cell oxidative stress orchestrates cell polarity to
promote embryonic wound healing. Dev Cell
47:377–387.e374
9. Ciruna B, Rossant J (2001) FGF signaling regulates mesoderm cell fate specification and
morphogenetic movement at the primitive
streak. Dev Cell 1(1):37–49
10. Tikhmyanova N, Golemis EA (2011) NEDD9
and BCAR1 negatively regulate E-cadherin
membrane
localization,
and
promote
E-cadherin degradation. PLoS One 6(7):
e22102. https://doi.org/10.1371/journal.
pone.0022102
11. Carvalho L, Jacinto A, Matova N (2014) The
Toll/NF-kappaB signaling pathway is required
for epidermal wound repair in Drosophila. Proc
Natl Acad Sci U S A 111:E5373–E5382.
https://doi.org/10.1073/pnas.1408224111
12. Hunter MV, Lee DM, Harris TJC, Gonzalez
RF (2015) Polarized E-cadherin endocytosis
directs actomyosin remodeling during embryonic wound repair. J Cell Biol 210:801–816.
https://doi.org/10.1083/jcb.201501076
13. Matsubayashi Y, Gilmer CC, Millard TH
(2015) Endocytosis-dependent coordination
of multiple actin regulators is required for
wound healing. J Cell Biol 210:5–7
14. Campbell K, Casanova J (2016) A common
framework for EMT and collective cell migration. Development 143:4291–4300. https://
doi.org/10.1242/dev.139071
15. Wood W, Jacinto A, Grose R, Woolner S,
Gale J, Wilson C, Martin P (2002) Wound
healing recapitulates morphogenesis in
Drosophila embryos. Nat Cell Biol 4:1–7.
https://doi.org/10.1038/ncb875
16. Abreu-Blanco MT, Verboon JM, Liu R, Watts
JJ, Parkhurst SM (2012) Drosophila embryos
close epithelial wounds using a combination of
cellular protrusions and an actomyosin purse
string. J Cell Sci 125:5984–5997. https://doi.
org/10.1242/jcs.109066
17. Hidalgo-Carcedo C, Hooper S, Chaudhry SI,
Williamson P, Harrington K, Leitinger B, Sahai
E (2011) Collective cell migration requires
suppression of actomyosin at cell-cell contacts
mediated by DDR1 and the cell polarity regulators Par3 and Par6. Nat Cell Biol 13
(1):49–58.
https://doi.org/10.1038/
ncb2133
18. Brock J, Midwinter K, Lewis J, Martin P
(1996) Healing of incisional wounds in the
embryonic chick wing bud: characterization of
the actin purse-string and demonstration of a
requirement for rho activation. J Cell Biol
135:1097–1107
19. Ducuing A, Vincent S (2016) The actin cable is
dispensable in directing dorsal closure dynamics but neutralizes mechanical stress to prevent
scarring in the Drosophila embryo. Nat Cell
Biol 18:1149–1160. https://doi.org/10.
1038/ncb3421
20. Zulueta-Coarasa T, Fernandez-Gonzalez R
(2018) Dynamic force patterns promote collective cell movements during embryonic
wound repair. Nat Phys 14:750–758. https://
doi.org/10.1038/s41567-018-0111-2
21. Oreopoulos J, Berman R, Browne M (2014)
Spinning-disk confocal microscopy: present
technology and future trends. In: Quantitative
imaging in cell biology, vol 123, 1st edn. Elsevier Inc., Amsterdam, pp 153–175. https://
doi.org/10.1016/B978-0-12-420138-5.
00009-4
22. Huisken J, Swoger J, Del Bene F, Wittbrodt J,
Stelzer EH (2004) Optical sectioning deep
inside live embryos by selective plane illumination
microscopy.
Science
305
(5686):1007–1009.
https://doi.org/10.
1126/science.1100035
23. Schmied C, Tomancak P (2016) Sample preparation and mounting of Drosophila embryos
for multiview light sheet microscopy. In: Drosophila: methods and protocols. Springer
New York, New York. https://doi.org/10.
1007/978-1-4939-6371-3_10
24. Gustafsson N, Culley S, Ashdown G, Owen
DM, Pereira PM, Henriques R (2016) Fast
live-cell conventional fluorophore nanoscopy
with ImageJ through super-resolution radial
fluctuations. Nat Commun 7:12471
222
Gordana Scepanovic et al.