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CEL.2018.05.014
26. Kiehart DP, Montague RA, Rickoll WL,
Foard D, Thomas GH (1994) High-resolution
microscopic methods for the analysis of cellular
movements in Drosophila embryos. In: Methods in cell biology, vol 44. Academic Press,
New York. https://doi.org/10.1016/S0091679X(08)60929-2
27. Edelstein A, Amodaj N, Hoover K, Vale R,
Stuurman N (2010) Computer control of
microscopes using μManager. Curr Protoc
Mol Biol 92:14.20.11–14.20.17. https://doi.
org/10.1002/0471142727.mb1420s92
28. Fernandez-Gonzalez R, Zallen J (2013)
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the early Drosophila embryo. Mol Biol Cell
24:3227–3237.
https://doi.org/10.1091/
mbc.E13-05-0228
29. Schindelin J, Arganda-Carreras I, Frise E,
Kaynig V, Longair M, Pietzsch T, Preibisch S,
Rueden C, Saalfeld S, Schmid B, Tinevez J-Y,
White DJ, Hartenstein V, Eliceiri K,
Tomancak P, Cardona A (2012) Fiji: an opensource platform for biological-image analysis.
Nat Methods 9:676–682. https://doi.org/10.
1038/nmeth.2019
30. Farge E (2003) Mechanical induction of Twist
in the Drosophila foregut/stomodeal primordium. Curr Biol 13:1365–1377. https://doi.
org/10.1016/s0960-9822(03)00576-1
31. Dijkstra EW (1959) A note on two problems in
connexion with graphs. Numer Math
1:269–271.
https://doi.org/10.1007/
BF01386390
32. Zulueta-Coarasa T, Tamada M, Lee EJ,
Fernandez-Gonzalez R (2014) Automated
multidimensional image analysis reveals a role
for Abl in embryonic wound repair. Development 141:2901–2911. https://doi.org/10.
1242/dev.106898
33. Sullivan W, Ashburner M, Hawley SR (2000)
Drosophila protocols. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
34. Kuntz SG, Eisen MB (2014) Drosophila
embryogenesis scales uniformly across temperature in developmentally diverse species. PLoS
Genet 10:e1004293. https://doi.org/10.
1371/journal.pgen.1004293
35. Culley S, Albrecht D, Jacobs C, Pereira PM,
Leterrier C, Mercer J, Henriques R (2018)
Quantitative mapping and minimization of
super-resolution optical imaging artifacts. Nat
Methods 15(4):263–266. https://doi.org/10.
1038/nmeth.4605
36. Cole RW, Jinadasa T, Brown CM (2011) Measuring and interpreting point spread functions
to determine confocal microscope resolution
and ensure quality control. Nat Protoc
6:1929–1941
37. Rauzi M, Lenne PF, Lecuit T (2010) Planar
polarized actomyosin contractile flows control
epithelial junction remodelling. Nature
468:1110–1115. https://doi.org/10.1038/
nature09566
38. Haack T, Schneider M, Schwendele B, Renault
AD (2014) Drosophila heart cell movement to
the midline occurs through both cell autonomous migration and dorsal closure. Dev Biol
396:169–182.
https://doi.org/10.1016/j.
ydbio.2014.08.033
39. Fre ´mion F, Astier M, Zaffran S, Guille `n A,
Homburger V, Se ´me ´riva M (1999) The heterotrimeric protein G is required for the formation
of heart epithelium in Drosophila. J Cell Biol
145:1063–1076. https://doi.org/10.1083/
jcb.145.5.1063
40. Huang J, Zhou W, Dong W, Watson AM,
Hong Y (2009) Directed, efficient, and versatile modifications of the Drosophila genome by
genomic engineering. Proc Natl Acad Sci U S A
106:8284–8289. https://doi.org/10.1073/
pnas.0900641106
41. Royou A, Field C, Sisson JC, Sullivan W, Karess
R (2003) Reassessing the role and dynamics of
nonmuscle myosin II during furrow formation
in early Drosophila embryos. Mol Biol Cell
15:838–850. https://doi.org/10.1091/mbc.
e03-06-0440
42. Martin AC, Kaschube M, Wieschaus EF (2009)
Pulsed contractions of an actin–myosin network drive apical constriction. Nature
457:495–499
43. Kiehart DP, Galbraith CG, Edwards KA, Rickoll WL, Montague RA (2000) Multiple forces
contribute to cell sheet morphogenesis for
dorsal closure in Drosophila. J Cell Biol
149:471–490
44. Rosales-Nieves AE, Johndrow JE, Keller LC,
Magie CR, Pinto-Santini DM, Parkhurst SM
(2006) Coordination of microtubule and
microfilament dynamics by Drosophila Rho1,
Spire and Cappuccino. Nat Cell Biol
8:367–376.
https://doi.org/10.1038/
ncb1385
45. Orsulic S, Peifer M (1996) An in vivo
structure-function study of armadillo, the
beta-catenin homologue, reveals both separate
and overlapping regions of the protein required
Live Imaging in Drosophila Embryos
223
101:74–79. https://doi.org/10.1016/J.BIO
CEL.2018.05.014
26. Kiehart DP, Montague RA, Rickoll WL,
Foard D, Thomas GH (1994) High-resolution
microscopic methods for the analysis of cellular
movements in Drosophila embryos. In: Methods in cell biology, vol 44. Academic Press,
New York. https://doi.org/10.1016/S0091679X(08)60929-2
27. Edelstein A, Amodaj N, Hoover K, Vale R,
Stuurman N (2010) Computer control of
microscopes using μManager. Curr Protoc
Mol Biol 92:14.20.11–14.20.17. https://doi.
org/10.1002/0471142727.mb1420s92
28. Fernandez-Gonzalez R, Zallen J (2013)
Wounded cells drive rapid epidermal repair in
the early Drosophila embryo. Mol Biol Cell
24:3227–3237.
https://doi.org/10.1091/
mbc.E13-05-0228
29. Schindelin J, Arganda-Carreras I, Frise E,
Kaynig V, Longair M, Pietzsch T, Preibisch S,
Rueden C, Saalfeld S, Schmid B, Tinevez J-Y,
White DJ, Hartenstein V, Eliceiri K,
Tomancak P, Cardona A (2012) Fiji: an opensource platform for biological-image analysis.
Nat Methods 9:676–682. https://doi.org/10.
1038/nmeth.2019
30. Farge E (2003) Mechanical induction of Twist
in the Drosophila foregut/stomodeal primordium. Curr Biol 13:1365–1377. https://doi.
org/10.1016/s0960-9822(03)00576-1
31. Dijkstra EW (1959) A note on two problems in
connexion with graphs. Numer Math
1:269–271.
https://doi.org/10.1007/
BF01386390
32. Zulueta-Coarasa T, Tamada M, Lee EJ,
Fernandez-Gonzalez R (2014) Automated
multidimensional image analysis reveals a role
for Abl in embryonic wound repair. Development 141:2901–2911. https://doi.org/10.
1242/dev.106898
33. Sullivan W, Ashburner M, Hawley SR (2000)
Drosophila protocols. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
34. Kuntz SG, Eisen MB (2014) Drosophila
embryogenesis scales uniformly across temperature in developmentally diverse species. PLoS
Genet 10:e1004293. https://doi.org/10.
1371/journal.pgen.1004293
35. Culley S, Albrecht D, Jacobs C, Pereira PM,
Leterrier C, Mercer J, Henriques R (2018)
Quantitative mapping and minimization of
super-resolution optical imaging artifacts. Nat
Methods 15(4):263–266. https://doi.org/10.
1038/nmeth.4605
36. Cole RW, Jinadasa T, Brown CM (2011) Measuring and interpreting point spread functions
to determine confocal microscope resolution
and ensure quality control. Nat Protoc
6:1929–1941
37. Rauzi M, Lenne PF, Lecuit T (2010) Planar
polarized actomyosin contractile flows control
epithelial junction remodelling. Nature
468:1110–1115. https://doi.org/10.1038/
nature09566
38. Haack T, Schneider M, Schwendele B, Renault
AD (2014) Drosophila heart cell movement to
the midline occurs through both cell autonomous migration and dorsal closure. Dev Biol
396:169–182.
https://doi.org/10.1016/j.
ydbio.2014.08.033
39. Fre ´mion F, Astier M, Zaffran S, Guille `n A,
Homburger V, Se ´me ´riva M (1999) The heterotrimeric protein G is required for the formation
of heart epithelium in Drosophila. J Cell Biol
145:1063–1076. https://doi.org/10.1083/
jcb.145.5.1063
40. Huang J, Zhou W, Dong W, Watson AM,
Hong Y (2009) Directed, efficient, and versatile modifications of the Drosophila genome by
genomic engineering. Proc Natl Acad Sci U S A
106:8284–8289. https://doi.org/10.1073/
pnas.0900641106
41. Royou A, Field C, Sisson JC, Sullivan W, Karess
R (2003) Reassessing the role and dynamics of
nonmuscle myosin II during furrow formation
in early Drosophila embryos. Mol Biol Cell
15:838–850. https://doi.org/10.1091/mbc.
e03-06-0440
42. Martin AC, Kaschube M, Wieschaus EF (2009)
Pulsed contractions of an actin–myosin network drive apical constriction. Nature
457:495–499
43. Kiehart DP, Galbraith CG, Edwards KA, Rickoll WL, Montague RA (2000) Multiple forces
contribute to cell sheet morphogenesis for
dorsal closure in Drosophila. J Cell Biol
149:471–490
44. Rosales-Nieves AE, Johndrow JE, Keller LC,
Magie CR, Pinto-Santini DM, Parkhurst SM
(2006) Coordination of microtubule and
microfilament dynamics by Drosophila Rho1,
Spire and Cappuccino. Nat Cell Biol
8:367–376.
https://doi.org/10.1038/
ncb1385
45. Orsulic S, Peifer M (1996) An in vivo
structure-function study of armadillo, the
beta-catenin homologue, reveals both separate
and overlapping regions of the protein required
Live Imaging in Drosophila Embryos
223