2.2 Mounting
Embryos
2.2.1 Glue Method
1. 50% bleach in tap water.
2. Mesh basket (Falcon, #352350, or equivalent).
3. Squirt water bottle.
4. Fine paper towels (Kimwipes, Kimtech Science, #KC34120, or
equivalent).
5. Fine-tip paint brush.
6. Razor blade.
7. A clean apple juice agar plate (with no yeast on the plate).
8. Cover slips, 18 Â 18 mm.
9. Heptane glue (see Note 2): heptane, double-sided tape, 20 mL
glass vial, and a Nutating Mixer.
10. A pair of forceps #55 (Dumont, #11295-51 or equivalent).
11. Glass Pasteur pipettes.
12. Halocarbon oil 27 and 700 mixed 1:1 in a 50 mL tube.
2.2.2 Teflon Membrane
Method
1. 50% bleach in tap water.
2. Mesh basket (Falcon, #352350, or equivalent).
3. Squirt water bottle.
4. Fine paper towels (Kimwipes, Kimtech Science, #KC34120, or
equivalent).
5. Fine-tip paint brush.
6. A clean apple juice agar plate (with no yeast on the plate).
7. Glass Pasteur pipettes.
8. Halocarbon oil 27 (see Note 3).
9. A pair of forceps #55 (Dumont, #11295-51, or equivalent).
10. Cover slips, 18 Â 18 mm.
11. An oxygen-permeable, teflon membrane set up on a mounting
slide [26].
2.3 Microinjection
1. 100 mm Petri dishes for embryo storage (Fig. 2a).
2. Small container filled with silica beads, covered with a small
cardboard platform (Fig. 2b).
3. Glass Pasteur pipettes, 150 mm.
4. Halocarbon oil 27 and 700 mixed 1:1 in a 50 mL tube.
5. 4-in., thin wall glass needle (WPI, #08E, or equivalent).
6. Needle puller (e.g., Sutter Instrument, Flaming/Brown
Micropippette Puller, #P-97 or equivalent).
7. 0.5–10 μL pipette.
8. Microloader tips.
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