nucleotide sequence. This plasmid was prepared by insertion between the ECO RI
and Hind III sites of pBR322.
Escherichia coli S17-1/pCRO1 rapidly decreased at 1-, 10-, or 100-times inoculation concentrations in viable counts using the selective media method, following
microcosm inoculation, and the abundance of microorganisms (in particular, its
primary consumer, the protozoan ciliate Cyclidium glaucoma) in the microcosm
increased. Escherichia coli S17-1/pCRO1 has been shown to be a suitable food
source for constituent heterotrophs in the microcosm. By comparison, in N 30 , no
significant difference was observed between the system with Escherichia coli added
and the system without it (control system). Moreover, a nearly identical behavior of
the introduced Escherichia coli S17-1/pCRO1, as a GEM, was observed in a natural
lake model ecosystem, that is, a naturally derived microcosm, which was made from
natural lake water including natural microorganisms and nutrients.
7.13.5 Pseudomonas aeruginosa PAO1/pCRO1
Pseudomonas aeruginosa PAO1/pCRO1 was added at 1, 10, and 100 times the
abundance of indigenous bacteria in the microcosm, and its influence on the structure and function of the ecosystem was examined. The pCRO1, with a 1580 bp
fragment size, is a mobilized transmissible plasmid through the chromosome, coding
Cr
r on its nucleotide sequence. This plasmid was prepared by insertion between the
ECO RI and Hind III sites of pBR322.
Pseudomonas aeruginosa PAO1/pCRO1 slowly decreased at 1-, 10-, or
100-times inoculation concentrations in viable counts using the selective media
method following microcosm inoculation, and the abundance of microorganisms
(in particular, its primary consumer, the protozoan ciliate Cyclidium glaucoma) in
the microcosm slowly increased. Pseudomonas aeruginosa PAO1/pCRO1 has been
shown to be a suitable food source for constituent heterotrophs in the microcosm. By
comparison, in N 30 , no significant difference was observed between the system with
Pseudomonas aeruginosa added and the system without it (control system). Moreover, a nearly identical behavior of the introduced Pseudomonas aeruginosa PAO1/
pCRO1, as a GEM, was observed in a natural lake model ecosystem, that is, a
naturally derived microcosm, which was made from natural lake water including
natural microorganisms and nutrients.
7.13.6 Pseudomonas putida PpY101/pSR134
Pseudomonas putida PpY101/pSR134 was added at 1, 10, and 100 times the abundance of indigenous bacteria in the microcosm, and its influence on the structure and
function of the ecosystem was examined. The pSR134 codes Hg
r on its nucleotide
sequence. Pseudomonas putida PpY101/pSR134 slowly decreased at 1-, 10-, or
142
K. Murakami et al.
and Hind III sites of pBR322.
Escherichia coli S17-1/pCRO1 rapidly decreased at 1-, 10-, or 100-times inoculation concentrations in viable counts using the selective media method, following
microcosm inoculation, and the abundance of microorganisms (in particular, its
primary consumer, the protozoan ciliate Cyclidium glaucoma) in the microcosm
increased. Escherichia coli S17-1/pCRO1 has been shown to be a suitable food
source for constituent heterotrophs in the microcosm. By comparison, in N 30 , no
significant difference was observed between the system with Escherichia coli added
and the system without it (control system). Moreover, a nearly identical behavior of
the introduced Escherichia coli S17-1/pCRO1, as a GEM, was observed in a natural
lake model ecosystem, that is, a naturally derived microcosm, which was made from
natural lake water including natural microorganisms and nutrients.
7.13.5 Pseudomonas aeruginosa PAO1/pCRO1
Pseudomonas aeruginosa PAO1/pCRO1 was added at 1, 10, and 100 times the
abundance of indigenous bacteria in the microcosm, and its influence on the structure and function of the ecosystem was examined. The pCRO1, with a 1580 bp
fragment size, is a mobilized transmissible plasmid through the chromosome, coding
Cr
r on its nucleotide sequence. This plasmid was prepared by insertion between the
ECO RI and Hind III sites of pBR322.
Pseudomonas aeruginosa PAO1/pCRO1 slowly decreased at 1-, 10-, or
100-times inoculation concentrations in viable counts using the selective media
method following microcosm inoculation, and the abundance of microorganisms
(in particular, its primary consumer, the protozoan ciliate Cyclidium glaucoma) in
the microcosm slowly increased. Pseudomonas aeruginosa PAO1/pCRO1 has been
shown to be a suitable food source for constituent heterotrophs in the microcosm. By
comparison, in N 30 , no significant difference was observed between the system with
Pseudomonas aeruginosa added and the system without it (control system). Moreover, a nearly identical behavior of the introduced Pseudomonas aeruginosa PAO1/
pCRO1, as a GEM, was observed in a natural lake model ecosystem, that is, a
naturally derived microcosm, which was made from natural lake water including
natural microorganisms and nutrients.
7.13.6 Pseudomonas putida PpY101/pSR134
Pseudomonas putida PpY101/pSR134 was added at 1, 10, and 100 times the abundance of indigenous bacteria in the microcosm, and its influence on the structure and
function of the ecosystem was examined. The pSR134 codes Hg
r on its nucleotide
sequence. Pseudomonas putida PpY101/pSR134 slowly decreased at 1-, 10-, or
142
K. Murakami et al.