7.13.2 Escherichia coli S17-1/pSUP104
Escherichia coli S17-1/pSUP104 was added at 1, 10, and 100 times the abundance
of indigenous bacteria in the microcosm, and its influence on the structure and
function of the ecosystem was examined. The pSUP104 is a mobilized transmissible
plasmid through the chromosome.
Escherichia coli S17-1/pSUP104 rapidly decreased at 1-, 10-, or 100-times
inoculation concentrations in viable counts using the selective media method,
following microcosm inoculation, and the abundance of microorganisms
(in particular, its primary consumer, Cyclidium glaucoma) in the microcosm
increased. Escherichia coli S17-1/pSUP104 has been shown to be a suitable food
source for constituent heterotrophs in the microcosm. By comparison, in N 30 , no
significant difference was observed between the system with Escherichia coli added
and the system without it (control system). Moreover, a nearly identical behavior of
introduced Escherichia coli S17-1/pSUP104, as a GEM, was observed in a natural
lake model ecosystem, that is, a naturally derived microcosm, which was made from
natural lake water including natural microorganisms and nutrients.
7.13.3 Escherichia coli C600/RP4
Escherichia coli C600/RP4 was added at 1, 10, and 100 times the abundance of
indigenous bacteria in the microcosm, and its influence on the structure and function
of the ecosystem was examined. The RP-4, 56-kbp-sized vector plasmid, which is
widely used for gene manipulation, is a self-transmissible plasmid, coding Ap
r , Tc
r ,
and Km
r and having an Eco RI site on its nucleotide sequence.
Escherichia coli C600/RP4 rapidly decreased at 1-, 10-, or 100-times inoculation
concentrations in viable counts using the selective media method, following microcosm inoculation, and the abundance of microorganisms (in particular, its primary
consumer, Cyclidium glaucoma) in the microcosm increased. Escherichia coli C600/
RP4 has been shown to be a suitable food source for constituent heterotrophs in the
microcosm. By comparison, in N 30 , no significant difference was observed between
the system with Escherichia coli added and the system without it (control system).
7.13.4 Escherichia coli S17-1/pCRO1
Escherichia coli S17-1/pCRO1 was added at 1, 10, and 100 times the abundance of
indigenous bacteria in the microcosm, and its influence on the structure and function
of the ecosystem was examined. The pCRO1, with a 1580 bp fragment size, is a
mobilized transmissible plasmid through the chromosome, coding Cr
r on its
7 Example Assessments of the Microcosm N-System
141
Escherichia coli S17-1/pSUP104 was added at 1, 10, and 100 times the abundance
of indigenous bacteria in the microcosm, and its influence on the structure and
function of the ecosystem was examined. The pSUP104 is a mobilized transmissible
plasmid through the chromosome.
Escherichia coli S17-1/pSUP104 rapidly decreased at 1-, 10-, or 100-times
inoculation concentrations in viable counts using the selective media method,
following microcosm inoculation, and the abundance of microorganisms
(in particular, its primary consumer, Cyclidium glaucoma) in the microcosm
increased. Escherichia coli S17-1/pSUP104 has been shown to be a suitable food
source for constituent heterotrophs in the microcosm. By comparison, in N 30 , no
significant difference was observed between the system with Escherichia coli added
and the system without it (control system). Moreover, a nearly identical behavior of
introduced Escherichia coli S17-1/pSUP104, as a GEM, was observed in a natural
lake model ecosystem, that is, a naturally derived microcosm, which was made from
natural lake water including natural microorganisms and nutrients.
7.13.3 Escherichia coli C600/RP4
Escherichia coli C600/RP4 was added at 1, 10, and 100 times the abundance of
indigenous bacteria in the microcosm, and its influence on the structure and function
of the ecosystem was examined. The RP-4, 56-kbp-sized vector plasmid, which is
widely used for gene manipulation, is a self-transmissible plasmid, coding Ap
r , Tc
r ,
and Km
r and having an Eco RI site on its nucleotide sequence.
Escherichia coli C600/RP4 rapidly decreased at 1-, 10-, or 100-times inoculation
concentrations in viable counts using the selective media method, following microcosm inoculation, and the abundance of microorganisms (in particular, its primary
consumer, Cyclidium glaucoma) in the microcosm increased. Escherichia coli C600/
RP4 has been shown to be a suitable food source for constituent heterotrophs in the
microcosm. By comparison, in N 30 , no significant difference was observed between
the system with Escherichia coli added and the system without it (control system).
7.13.4 Escherichia coli S17-1/pCRO1
Escherichia coli S17-1/pCRO1 was added at 1, 10, and 100 times the abundance of
indigenous bacteria in the microcosm, and its influence on the structure and function
of the ecosystem was examined. The pCRO1, with a 1580 bp fragment size, is a
mobilized transmissible plasmid through the chromosome, coding Cr
r on its
7 Example Assessments of the Microcosm N-System
141