100-times inoculation concentrations in viable counts using the selective media
method, following microcosm inoculation, and the abundance of microorganisms
(in particular, its primary consumer, the protozoan ciliate Cyclidium glaucoma) in
the microcosm slowly increased. Pseudomonas putida PpY101/pSR134 has been
shown to be a suitable food source for constituent heterotrophs in the microcosm.
By comparison, in N 30 , no significant difference was observed between the system
with Pseudomonas putida added and the system without it (control system).
7.13.7 Pseudomonas putida MC/pBR325
Pseudomonas putida MC/pBR325 was added at 1, 10, and 100 times the abundance
of indigenous bacteria in the microcosm, and its influence on the structure and
function of the ecosystem was examined. Pseudomonas putida MC is one of the
indigenous bacteria in the microcosm N-system. The pBR325, a 5.9-kbp-sized
vector plasmid, which is widely used for gene manipulation, is a non-transmissible
plasmid, coding Cm
r , Tc
r , and Ap
r and having Eco RI, Bam HI, Hind III, and Sal
I sites on its nucleotide sequence. Pseudomonas putida MC/pBR325 slowly
decreased at 1-, 10-, or 100-times inoculation concentrations in viable counts
using the selective media method after microcosm inoculation, and the abundance
of microorganisms (in particular, its primary consumer, the protozoan ciliate
Cyclidium glaucoma) in the microcosm slowly increased. Pseudomonas putida
MC/pBR325 has been shown to be a suitable food source for constituent heterotrophs in the microcosm. By comparison, in N 30 , no significant difference was
observed between the system with Pseudomonas putida added and the system
without it (control system).
7.13.8 Pseudomonas paucimobilis BHC+
Pseudomonas paucimobilis BHC+ was added at 1, 10, and 100 times the abundance
of indigenous bacteria in the microcosm, and its influence on the structure and
function of the ecosystem was examined. Pseudomonas paucimobilis is a BHC+degrading bacterium. Pseudomonas paucimobilis BHC+ slowly decreased at 1-, 10-,
or 100-times inoculation concentrations in viable counts using the selective media
method after microcosm inoculation, and the abundance of microorganisms
(in particular, its primary consumer, protozoan ciliate Cyclidium glaucoma) in the
microcosm slowly increased. Pseudomonas paucimobilis BHC+ has been shown to
be a suitable food source for constituent heterotrophs in the microcosm. By comparison, in N 30 , no significant difference was observed between the system with
Pseudomonas paucimobilis added and the system without it (control system).
7 Example Assessments of the Microcosm N-System
143
method, following microcosm inoculation, and the abundance of microorganisms
(in particular, its primary consumer, the protozoan ciliate Cyclidium glaucoma) in
the microcosm slowly increased. Pseudomonas putida PpY101/pSR134 has been
shown to be a suitable food source for constituent heterotrophs in the microcosm.
By comparison, in N 30 , no significant difference was observed between the system
with Pseudomonas putida added and the system without it (control system).
7.13.7 Pseudomonas putida MC/pBR325
Pseudomonas putida MC/pBR325 was added at 1, 10, and 100 times the abundance
of indigenous bacteria in the microcosm, and its influence on the structure and
function of the ecosystem was examined. Pseudomonas putida MC is one of the
indigenous bacteria in the microcosm N-system. The pBR325, a 5.9-kbp-sized
vector plasmid, which is widely used for gene manipulation, is a non-transmissible
plasmid, coding Cm
r , Tc
r , and Ap
r and having Eco RI, Bam HI, Hind III, and Sal
I sites on its nucleotide sequence. Pseudomonas putida MC/pBR325 slowly
decreased at 1-, 10-, or 100-times inoculation concentrations in viable counts
using the selective media method after microcosm inoculation, and the abundance
of microorganisms (in particular, its primary consumer, the protozoan ciliate
Cyclidium glaucoma) in the microcosm slowly increased. Pseudomonas putida
MC/pBR325 has been shown to be a suitable food source for constituent heterotrophs in the microcosm. By comparison, in N 30 , no significant difference was
observed between the system with Pseudomonas putida added and the system
without it (control system).
7.13.8 Pseudomonas paucimobilis BHC+
Pseudomonas paucimobilis BHC+ was added at 1, 10, and 100 times the abundance
of indigenous bacteria in the microcosm, and its influence on the structure and
function of the ecosystem was examined. Pseudomonas paucimobilis is a BHC+degrading bacterium. Pseudomonas paucimobilis BHC+ slowly decreased at 1-, 10-,
or 100-times inoculation concentrations in viable counts using the selective media
method after microcosm inoculation, and the abundance of microorganisms
(in particular, its primary consumer, protozoan ciliate Cyclidium glaucoma) in the
microcosm slowly increased. Pseudomonas paucimobilis BHC+ has been shown to
be a suitable food source for constituent heterotrophs in the microcosm. By comparison, in N 30 , no significant difference was observed between the system with
Pseudomonas paucimobilis added and the system without it (control system).
7 Example Assessments of the Microcosm N-System
143