(b) Boil the agar and then add total measure of bean powder and
stir well.
(c) After that, add all the powder and liquid forms of constituents
to the blender and mix well.
(d) Dispense the ready diets in the preferred number of sterilized
plastic boxes, and permit for cooling and hardening.
1.3.3 Procedure
1. Collect the eggs from primary sources.
2. Place the eggs on the wax paper and keep on the surface of the
diet (200 g) in the container with aeration.
3. Egg hatches into larvae (first instars), looks like a tiny threadlike structure, colorless, and after 15 days, transforms into
second instars.
4. Place the third instar larvae on new container with diet to
undergo fourth and fifth stages.
5. Use fifth or late instar larvae for experimental studies.
6. Allow the larva to pupate in the dry container.
7. Collect the pupae and keep in the petri plates containing cotton
wool and transfer to oviposition cages for adult emergence.
8. A pair of male and female is positioned in a rearing box for
laying eggs. Sucrose solution saturated in cotton wool is positioned in a cup for adults feeding. A piece of wax paper is
inserted in the box on which the female lays eggs.
9. Collect egg sheets daily and place in clean containers.
2 Collection and Processing of Soil Samples
2.1 Soil Samples
Soil samplings can be done from natural environments like agricultural fields, riverbanks, forests, meadows, or pastures (non- or
low-human-disturbed) orchards, and manure heaps. EPNs are
identified from a diversity of soil habitats, and the numerous species
and isolates display substantial difference in host distribution, proliferation, infectivity, and survival conditions (i.e., temperature, soil
moisture).
Soil is a tremendous locale for nematodes, and 100 Cc (coefficient of curvature) of soil could encompass a hundred thousand of
them. Because of their prominence to agriculture, many more is
learned about plant-parasitic nematodes than the other categories
of nematodes in soil. Many soil nematodes do not parasitize plants,
but are advantageous in the breakdown of organic matter. These
nematodes are also denoted as free-living nematodes. In soil, juvenile or other stages of animal and insect parasites can also be
established. While certain plant parasites may live inside the roots
of the plant, most nematodes occupy the thin film of moisture near
Collection and Processing of Soil Samples
55
stir well.
(c) After that, add all the powder and liquid forms of constituents
to the blender and mix well.
(d) Dispense the ready diets in the preferred number of sterilized
plastic boxes, and permit for cooling and hardening.
1.3.3 Procedure
1. Collect the eggs from primary sources.
2. Place the eggs on the wax paper and keep on the surface of the
diet (200 g) in the container with aeration.
3. Egg hatches into larvae (first instars), looks like a tiny threadlike structure, colorless, and after 15 days, transforms into
second instars.
4. Place the third instar larvae on new container with diet to
undergo fourth and fifth stages.
5. Use fifth or late instar larvae for experimental studies.
6. Allow the larva to pupate in the dry container.
7. Collect the pupae and keep in the petri plates containing cotton
wool and transfer to oviposition cages for adult emergence.
8. A pair of male and female is positioned in a rearing box for
laying eggs. Sucrose solution saturated in cotton wool is positioned in a cup for adults feeding. A piece of wax paper is
inserted in the box on which the female lays eggs.
9. Collect egg sheets daily and place in clean containers.
2 Collection and Processing of Soil Samples
2.1 Soil Samples
Soil samplings can be done from natural environments like agricultural fields, riverbanks, forests, meadows, or pastures (non- or
low-human-disturbed) orchards, and manure heaps. EPNs are
identified from a diversity of soil habitats, and the numerous species
and isolates display substantial difference in host distribution, proliferation, infectivity, and survival conditions (i.e., temperature, soil
moisture).
Soil is a tremendous locale for nematodes, and 100 Cc (coefficient of curvature) of soil could encompass a hundred thousand of
them. Because of their prominence to agriculture, many more is
learned about plant-parasitic nematodes than the other categories
of nematodes in soil. Many soil nematodes do not parasitize plants,
but are advantageous in the breakdown of organic matter. These
nematodes are also denoted as free-living nematodes. In soil, juvenile or other stages of animal and insect parasites can also be
established. While certain plant parasites may live inside the roots
of the plant, most nematodes occupy the thin film of moisture near
Collection and Processing of Soil Samples
55