1.3.2 Rearing
Materials Required
l
Wax paper.
l
Artificial diet.
l
Cotton wool.
l
Plastic container.
l
Eggs.
Preparation of Diet
l
Kidney bean flour: 200 g.
l
Yeast powder: 30 g.
l
Ascorbic acid: 3.5 g.
l
Methyl-p-hydroxybenzoate: 2 g.
l
Sorbic acid: 1 g.
l
Formaldehyde solution: 2.5 ml.
l
Agar: 14 g.
l
Distilled water: 500 ml.
(a) Weigh and measure cautiously the powder and liquid forms of
constituents of the diet and keep in distinct containers.
Fig. 3 Life cycle of Spodoptera litura
54
Laboratory Techniques for Entomopathogenic Nematodes
Materials Required
l
Wax paper.
l
Artificial diet.
l
Cotton wool.
l
Plastic container.
l
Eggs.
Preparation of Diet
l
Kidney bean flour: 200 g.
l
Yeast powder: 30 g.
l
Ascorbic acid: 3.5 g.
l
Methyl-p-hydroxybenzoate: 2 g.
l
Sorbic acid: 1 g.
l
Formaldehyde solution: 2.5 ml.
l
Agar: 14 g.
l
Distilled water: 500 ml.
(a) Weigh and measure cautiously the powder and liquid forms of
constituents of the diet and keep in distinct containers.
Fig. 3 Life cycle of Spodoptera litura
54
Laboratory Techniques for Entomopathogenic Nematodes