General Note
For optimization of HPLC separation several different parameters can be used:
particle size and porosity, variation of interaction and forces between analytes
and stationary phase, gradient elution and some more. All parameters influence chromatographic resolution and efficiency.
Pump
Pumps
mixing chamber
Eluents
Column
Binary gradient system
Ɵme
% B in A
linear gradient
non-linear gradient
Fig. 4.18 Binary gradient
system as used in HPLC
(adopted from and
simplified after Schwedt
2007)
58
4 Instrumental Analysis
For optimization of HPLC separation several different parameters can be used:
particle size and porosity, variation of interaction and forces between analytes
and stationary phase, gradient elution and some more. All parameters influence chromatographic resolution and efficiency.
Pump
Pumps
mixing chamber
Eluents
Column
Binary gradient system
Ɵme
% B in A
linear gradient
non-linear gradient
Fig. 4.18 Binary gradient
system as used in HPLC
(adopted from and
simplified after Schwedt
2007)
58
4 Instrumental Analysis