56
3 Study of Electrophysical Characteristics of Blood …
such as the real and imaginary parts of their refractive indices, dimensions, etc. This
formula cannot be further simplified without loss of information, and, hence, the
relations between the mode frequencies and parameters of the medium and resonator
should be analyzed numerically.
The model is implemented in a software package that allows automatic variations
in the measured real and imaginary parts of the refractive indices and sizes of particles
on a single setup. Such an approach makes it possible to reveal correlations of the
electrophysical parameters and biological properties of blood formed elements. The
optical parameters of blood cells must substantially supplement a detailed analysis
of blood owing to more precise characterization of the cells. The model for the
estimation of the refractive indices and sizes of blood formed elements and the
intracavity measurements can be more informative and accurate in comparison with
the existing methods that employ cavity-free models.
3.8 Numerical Calculations for the Resonator
with a Simulated Medium and Conclusions
A very important area of application of laser radiation is biomedical optics. Using
optical techniques, one can study mechanisms behind the interaction of cells with
the environment and their response to changes in the physical properties of the
medium and thereby gain information about an ensemble of living cells, including
blood corpuscles (hemocytes), which play a key role in different physicochemical
interactions. The advantage of laser radiation (laser beam) in studying biological
particles is that it does not cause crude pathomorphological changes in the tissue. At
the same time, laser diagnostics effectively utilizes such properties of laser radiation
as coherency, monochromaticity, and directionality. Development of new methods
of laser biomedical diagnostics requires a theoretical analysis of light propagation
in biological tissues. The presence of an adequate theory will make it possible to
better appreciate optical measurement data and raise the potential, reliability, and
usefulness of optical technologies.
It is believed that such an approach will expand the information content of the
intracavity method in analyzing the dependences of the optical characteristics on the
radiation wavelength. In this work, we simulate the absorption versus the wavelength
dependence for different parameters of the medium being simulated. The simulation
demonstrates the feasibility of this method for studying biological structures of various configurations, such as an ensemble of spherulated particles with a nonconcentric
inclusion (hemocyte suspensions). Erythrocytes have largely a spherical doubly concave shape. However, in general, the shape of an erythrocyte depends on intracellular
factors and the environment. Sometimes, erythrocytes may be spherical, for example,
when the cell is in a hypotonic solution.
3 Study of Electrophysical Characteristics of Blood …
such as the real and imaginary parts of their refractive indices, dimensions, etc. This
formula cannot be further simplified without loss of information, and, hence, the
relations between the mode frequencies and parameters of the medium and resonator
should be analyzed numerically.
The model is implemented in a software package that allows automatic variations
in the measured real and imaginary parts of the refractive indices and sizes of particles
on a single setup. Such an approach makes it possible to reveal correlations of the
electrophysical parameters and biological properties of blood formed elements. The
optical parameters of blood cells must substantially supplement a detailed analysis
of blood owing to more precise characterization of the cells. The model for the
estimation of the refractive indices and sizes of blood formed elements and the
intracavity measurements can be more informative and accurate in comparison with
the existing methods that employ cavity-free models.
3.8 Numerical Calculations for the Resonator
with a Simulated Medium and Conclusions
A very important area of application of laser radiation is biomedical optics. Using
optical techniques, one can study mechanisms behind the interaction of cells with
the environment and their response to changes in the physical properties of the
medium and thereby gain information about an ensemble of living cells, including
blood corpuscles (hemocytes), which play a key role in different physicochemical
interactions. The advantage of laser radiation (laser beam) in studying biological
particles is that it does not cause crude pathomorphological changes in the tissue. At
the same time, laser diagnostics effectively utilizes such properties of laser radiation
as coherency, monochromaticity, and directionality. Development of new methods
of laser biomedical diagnostics requires a theoretical analysis of light propagation
in biological tissues. The presence of an adequate theory will make it possible to
better appreciate optical measurement data and raise the potential, reliability, and
usefulness of optical technologies.
It is believed that such an approach will expand the information content of the
intracavity method in analyzing the dependences of the optical characteristics on the
radiation wavelength. In this work, we simulate the absorption versus the wavelength
dependence for different parameters of the medium being simulated. The simulation
demonstrates the feasibility of this method for studying biological structures of various configurations, such as an ensemble of spherulated particles with a nonconcentric
inclusion (hemocyte suspensions). Erythrocytes have largely a spherical doubly concave shape. However, in general, the shape of an erythrocyte depends on intracellular
factors and the environment. Sometimes, erythrocytes may be spherical, for example,
when the cell is in a hypotonic solution.