Shan Q, Wang Y, Li J, Zhang Y, Chen K, Liang Z, Zhang K, Liu J, Xi JJ, Qiu JL, Gao C (2013)
Targeted genome modification of crop plants using a CRISPR-Cas system. Nat Biotechnol
31(8):686–688. https://doi.org/10.1038/nbt.2650
Shoji Y, Chichester JA, Palmer GA, Farrance CE, Stevens R, Stewart MGoldschmidt L, Deyde V,
Gubareva L, Klimov A, Mett V, Yusibov V (2011) An influenza N1 neuraminidase-specific
monoclonal antibody with broad neuraminidase inhibition activity against H5N1 HPAI viruses.
Hum Vaccin 7(Suppl):199–204
Shoji Y, Jones RM, Mett V, Chichester JA, Musiychuk K, Sun X, Tumpey TM, Green BJ,
Shamloul M, Norikane J, Bi H, Hartman CE, Bottone C, Stewart M, Streatfield SJ,
Yusibov V (2013) A plant-produced H1N1 trimeric hemagglutinin protects mice from a lethal
influenza virus challenge. Hum Vaccin Immunother 9(3):553–560
Shoji Y, Prokhnevsky A, Leffet B, Vetter N, Tottey S, Satinover S, Musiychuk K, Shamloul M,
Norikane J, Jones RM, Chichester JA, Green BJ, Streatfield SJ, Yusibov V (2015) Immunogenicity of H1N1 influenza virus-like particles produced in Nicotiana benthamiana. Hum Vaccin
Immunother 11(1):118–123. https://doi.org/10.4161/hv.34365
Simmons G, Wool-Lewis RJ, Baribaud F, Netter RC, Bates P (2002) Ebola virus glycoproteins
induce global surface protein down-modulation and loss of cell adherence. J Virol
76:2518–2528
Singh A, Srivastava S, Chouksey A, Panwar BS, Verma PC, Roy S, Singh PK, Saxena G, Tuli R
(2015) Expression of rabies glycoprotein and ricin toxin B chain (RGP-RTB) fusion protein in
tomato hairy roots: a step towards oral vaccination for rabies. Mol Biotechnol 57(4):359–370.
https://doi.org/10.1007/s12033-014-9829-y
Skarjinskaia M, Ruby K, Araujo A, Taylor K, Gopalasamy-Raju V, Musiychuk K, Chichester JA,
Palmer GA, de la Rosa P, Mett V, Ugulava N, Streatfield SJ, Yusibov V (2013) Hairy roots as
a vaccine production and delivery system. Adv Biochem Eng Biotechnol 134:115–134. https://
doi.org/10.1007/10_2013_184
Sparrow P, Broer I, Hood EE, Eversole K, Hartung F, Schiemann J (2013) Risk assessment
and regulation of molecular farming - a comparison between Europe and US. Curr Pharm Des
19(31):5513–5530
Strasser R, Stadlmann J, Schahs M, Stiegler G, Quendler H, Mach L, Glössl J, Weterings K,
Pabst M, Steinkellner H (2008) Generation of glyco-engineered Nicotiana benthamiana for
the production of monoclonal antibodies with a homogeneous human-like N-glycan structure.
Plant Biotechnol J 6(4):392–402. https://doi.org/10.1111/j.1467-7652.2008.00330.x
Streatfield SJ (2006) Mucosal immunization using recombinant plant-based oral vaccines. Methods
38(2):150–157. https://doi.org/10.1016/j.ymeth.2005.09.013
Tacket CO (2007) Plant-based vaccines against diarrheal diseases. Trans Am Clin Climatol Assoc
118:79–87
Tacket CO, Mason HS, Losonsky G, Clements JD, Levine MM, Arntzen CJ (1998) Immunogenicity in humans of a recombinant bacterial antigen delivered in a transgenic potato. Nat Med
4(5):607–609
Tacket CO, Mason HS, Losonsky G, Estes MK, Levine MM, Arntzen CJ (2000) Human immune
responses to a novel Norwalk virus vaccine delivered in transgenic potatoes. J Infect Dis
182(1):302–305. https://doi.org/10.1086/315653
Takeyama N, Kiyono H, Yuki Y (2015a) Plant-based vaccines for animals and humans: recent
advances in technology and clinical trials. Ther Adv Vaccin 3(5–6):139–154. https://doi.org/10.
1177/2051013615613272
Takeyama N, Yuki Y, Tokuhara D, Oroku K, Mejima M, Kurokawa S, Kuroda M, Kodama T,
Nagai S, Ueda S, Kiyono H (2015b) Oral rice-based vaccine induces passive and
active immunity against enterotoxigenic E. coli-mediated diarrhea in pigs. Vaccine
33(39):5204–5211. https://doi.org/10.1016/j.vaccine.2015.07.074
Thanavala Y, Mahoney M, Pal S, Scott A, Richter L, Natarajan N, Goodwin P, Arntzen CJ,
Mason HS (2005) Immunogenicity in humans of an edible vaccine for hepatitis B. Proc Natl
Acad Sci U S A 102(9):3378–3382. https://doi.org/10.1073/pnas.0409899102
Tinland B, Hohn B (1995) Recombination between prokaryotic and eukaryotic DNA: integration
of Agrobacterium tumefaciens T-DNA into the plant genome. Genet Eng (NY) 17:209–229
106
S. Massa et al.
Targeted genome modification of crop plants using a CRISPR-Cas system. Nat Biotechnol
31(8):686–688. https://doi.org/10.1038/nbt.2650
Shoji Y, Chichester JA, Palmer GA, Farrance CE, Stevens R, Stewart MGoldschmidt L, Deyde V,
Gubareva L, Klimov A, Mett V, Yusibov V (2011) An influenza N1 neuraminidase-specific
monoclonal antibody with broad neuraminidase inhibition activity against H5N1 HPAI viruses.
Hum Vaccin 7(Suppl):199–204
Shoji Y, Jones RM, Mett V, Chichester JA, Musiychuk K, Sun X, Tumpey TM, Green BJ,
Shamloul M, Norikane J, Bi H, Hartman CE, Bottone C, Stewart M, Streatfield SJ,
Yusibov V (2013) A plant-produced H1N1 trimeric hemagglutinin protects mice from a lethal
influenza virus challenge. Hum Vaccin Immunother 9(3):553–560
Shoji Y, Prokhnevsky A, Leffet B, Vetter N, Tottey S, Satinover S, Musiychuk K, Shamloul M,
Norikane J, Jones RM, Chichester JA, Green BJ, Streatfield SJ, Yusibov V (2015) Immunogenicity of H1N1 influenza virus-like particles produced in Nicotiana benthamiana. Hum Vaccin
Immunother 11(1):118–123. https://doi.org/10.4161/hv.34365
Simmons G, Wool-Lewis RJ, Baribaud F, Netter RC, Bates P (2002) Ebola virus glycoproteins
induce global surface protein down-modulation and loss of cell adherence. J Virol
76:2518–2528
Singh A, Srivastava S, Chouksey A, Panwar BS, Verma PC, Roy S, Singh PK, Saxena G, Tuli R
(2015) Expression of rabies glycoprotein and ricin toxin B chain (RGP-RTB) fusion protein in
tomato hairy roots: a step towards oral vaccination for rabies. Mol Biotechnol 57(4):359–370.
https://doi.org/10.1007/s12033-014-9829-y
Skarjinskaia M, Ruby K, Araujo A, Taylor K, Gopalasamy-Raju V, Musiychuk K, Chichester JA,
Palmer GA, de la Rosa P, Mett V, Ugulava N, Streatfield SJ, Yusibov V (2013) Hairy roots as
a vaccine production and delivery system. Adv Biochem Eng Biotechnol 134:115–134. https://
doi.org/10.1007/10_2013_184
Sparrow P, Broer I, Hood EE, Eversole K, Hartung F, Schiemann J (2013) Risk assessment
and regulation of molecular farming - a comparison between Europe and US. Curr Pharm Des
19(31):5513–5530
Strasser R, Stadlmann J, Schahs M, Stiegler G, Quendler H, Mach L, Glössl J, Weterings K,
Pabst M, Steinkellner H (2008) Generation of glyco-engineered Nicotiana benthamiana for
the production of monoclonal antibodies with a homogeneous human-like N-glycan structure.
Plant Biotechnol J 6(4):392–402. https://doi.org/10.1111/j.1467-7652.2008.00330.x
Streatfield SJ (2006) Mucosal immunization using recombinant plant-based oral vaccines. Methods
38(2):150–157. https://doi.org/10.1016/j.ymeth.2005.09.013
Tacket CO (2007) Plant-based vaccines against diarrheal diseases. Trans Am Clin Climatol Assoc
118:79–87
Tacket CO, Mason HS, Losonsky G, Clements JD, Levine MM, Arntzen CJ (1998) Immunogenicity in humans of a recombinant bacterial antigen delivered in a transgenic potato. Nat Med
4(5):607–609
Tacket CO, Mason HS, Losonsky G, Estes MK, Levine MM, Arntzen CJ (2000) Human immune
responses to a novel Norwalk virus vaccine delivered in transgenic potatoes. J Infect Dis
182(1):302–305. https://doi.org/10.1086/315653
Takeyama N, Kiyono H, Yuki Y (2015a) Plant-based vaccines for animals and humans: recent
advances in technology and clinical trials. Ther Adv Vaccin 3(5–6):139–154. https://doi.org/10.
1177/2051013615613272
Takeyama N, Yuki Y, Tokuhara D, Oroku K, Mejima M, Kurokawa S, Kuroda M, Kodama T,
Nagai S, Ueda S, Kiyono H (2015b) Oral rice-based vaccine induces passive and
active immunity against enterotoxigenic E. coli-mediated diarrhea in pigs. Vaccine
33(39):5204–5211. https://doi.org/10.1016/j.vaccine.2015.07.074
Thanavala Y, Mahoney M, Pal S, Scott A, Richter L, Natarajan N, Goodwin P, Arntzen CJ,
Mason HS (2005) Immunogenicity in humans of an edible vaccine for hepatitis B. Proc Natl
Acad Sci U S A 102(9):3378–3382. https://doi.org/10.1073/pnas.0409899102
Tinland B, Hohn B (1995) Recombination between prokaryotic and eukaryotic DNA: integration
of Agrobacterium tumefaciens T-DNA into the plant genome. Genet Eng (NY) 17:209–229
106
S. Massa et al.