elemental mapping of biological specimens with a dual-EDS dedicated scanning transmission electron. Ultramicroscopy 128, 24–31 (2013). doi:10.1016/j.ultramic.2013.01.004
39. Y.H. Pan, K. Sader, J.J. Powell, A. Bleloch, M. Gass, J. Trinick, A. Warley, A. Li, R.
Brydson, A. Brown, 3D morphology of the human hepatic ferritin mineral core: new
evidence for a subunit structure revealed by single particle analysis of HAADF-STEM
images. J. Struct. Biol. 166(1), 22–31 (2009). doi:10.1016/j.jsb.2008.12.001
40. F.S. Sjostrand, Ultrastructure of retinal rod synapses of the guinea pig eye as revealed by
three-dimensional reconstructions from serial sections. J. Ultrastruct. Res. 2(1), 122–170
(1958)
41. F. Nagata, K. Hama, K.R. Porter, Three dimensional observation of biological specimen
with high voltage electron microscope. J. Electron. Microsc. (Tokyo) 18(2), 106–109 (1969)
42. A.M. Glauert, The high voltage electron microscope in biology. J. Cell Biol. 63(3), 717–748
(1974)
43. P. Favard, N. Carasso, The preparation and observation of thick biological sections in the
high voltage electron microscope. J. Microsc. 97(1), 59–81 (1973)
44. G.L. Scott, R.W. Guillery, Studies with the high voltage electron microscope of normal,
degenerating, and Golgi impregnated neuronal processes. J. Neurocytol. 3(5), 567–590
(1974)
45. T. Arii, K. Hama, Method of extracting three-dimensional information from HVTEM stereo
images of biological materials. J. Electron. Microsc. (Tokyo) 36(4), 177–195 (1987)
46. J. Frank, Electron tomography: methods for three-dimensional visualization of structures in
the cell, 2nd edn. (Springer, New York, London, 2006)
47. A. Takaoka, T. Hasegawa, K. Yoshida, H. Mori, Microscopic tomography with ultra-HVEM
and applications. Ultramicroscopy 108(3), 230–238 (2008). doi:10.1016/j.ultramic.2007.06.
008
48. K. Murata, M. Esaki, T. Ogura, S. Arai, Y. Yamamoto, N. Tanaka, Whole-cell imaging of
the budding yeast Saccharomyces cerevisiae by high-voltage scanning transmission electron
tomography. Ultramicroscopy 146, 39–45 (2014). doi:10.1016/j.ultramic.2014.05.008
49. M. vonArdenne, Personal recollections about the development of the electron microscope
and its technology. Optik 105(4), 139–153 (1997)
50. T. Groves, Thick specimens in the CEM and STEM resolution and image formation.
Ultramicroscopy 1(15–31) (1975)
51. K. Zierold, X-ray microanalysis of freeze-dried and frozen-hydrated cryosections. J Electron
Microsc. Tech. 9(1), 65–82 (1988). doi:10.1002/jemt.1060090107
52. R.D. Leapman, Detecting single atoms of calcium and iron in biological structures by
electron energy-loss spectrum-imaging. J. Microsc. 210(1), 5–15 (2003). doi:10.1046/j.
1365-2818.2003.01173.x
53. M.T. Otten, D.J. Stenzel, D.R. Cousens, B.M. Humbel, J.L.M. Leunissen, Y.D. Stierhof, W.
M. Busing, High-angle annular dark-field STEM imaging of immunogold labels. Scanning
14(5), 282–289 (1992). doi:10.1002/sca.4950140507
54. U. Ziese, C. Kubel, A. Verkleij, A.J. Koster, Three-dimensional localization of ultrasmall
immuno-gold labels by HAADF-STEM tomography. J. Struct. Biol. 138(1–2), 58–62
(2002). doi:10.1016/S1047-8477(02)00018-7
55. A.A. Sousa, M.A. Aronova, Y.C. Kim, L.M. Dorward, G. Zhang, R.D. Leapman, On the
feasibility of visualizing ultrasmall gold labels in biological specimens by STEM
tomography. J. Struct. Biol. 159(3), 507–522 (2007). doi:10.1016/j.jsb.2007.06.006
56. P. Gentsch, H. Gilde, L. Reimer, Measurement of the top bottom effect in scanning
transmission electron microscopy of thick amorphous specimens. J. Microsc. 100(1), 81–92
(1974). doi:10.1111/j.1365-2818.1974.tb03915.x
57. D.J. Smith, J.M. Cowley, Aperture contrast in thick amorphous specimens using scanning
transmission electron microscopy. Ultramicroscopy 1(2), 127–136 (1975). doi:10.1111/epi.
12023
2 STEM Tomography in Biology
57
39. Y.H. Pan, K. Sader, J.J. Powell, A. Bleloch, M. Gass, J. Trinick, A. Warley, A. Li, R.
Brydson, A. Brown, 3D morphology of the human hepatic ferritin mineral core: new
evidence for a subunit structure revealed by single particle analysis of HAADF-STEM
images. J. Struct. Biol. 166(1), 22–31 (2009). doi:10.1016/j.jsb.2008.12.001
40. F.S. Sjostrand, Ultrastructure of retinal rod synapses of the guinea pig eye as revealed by
three-dimensional reconstructions from serial sections. J. Ultrastruct. Res. 2(1), 122–170
(1958)
41. F. Nagata, K. Hama, K.R. Porter, Three dimensional observation of biological specimen
with high voltage electron microscope. J. Electron. Microsc. (Tokyo) 18(2), 106–109 (1969)
42. A.M. Glauert, The high voltage electron microscope in biology. J. Cell Biol. 63(3), 717–748
(1974)
43. P. Favard, N. Carasso, The preparation and observation of thick biological sections in the
high voltage electron microscope. J. Microsc. 97(1), 59–81 (1973)
44. G.L. Scott, R.W. Guillery, Studies with the high voltage electron microscope of normal,
degenerating, and Golgi impregnated neuronal processes. J. Neurocytol. 3(5), 567–590
(1974)
45. T. Arii, K. Hama, Method of extracting three-dimensional information from HVTEM stereo
images of biological materials. J. Electron. Microsc. (Tokyo) 36(4), 177–195 (1987)
46. J. Frank, Electron tomography: methods for three-dimensional visualization of structures in
the cell, 2nd edn. (Springer, New York, London, 2006)
47. A. Takaoka, T. Hasegawa, K. Yoshida, H. Mori, Microscopic tomography with ultra-HVEM
and applications. Ultramicroscopy 108(3), 230–238 (2008). doi:10.1016/j.ultramic.2007.06.
008
48. K. Murata, M. Esaki, T. Ogura, S. Arai, Y. Yamamoto, N. Tanaka, Whole-cell imaging of
the budding yeast Saccharomyces cerevisiae by high-voltage scanning transmission electron
tomography. Ultramicroscopy 146, 39–45 (2014). doi:10.1016/j.ultramic.2014.05.008
49. M. vonArdenne, Personal recollections about the development of the electron microscope
and its technology. Optik 105(4), 139–153 (1997)
50. T. Groves, Thick specimens in the CEM and STEM resolution and image formation.
Ultramicroscopy 1(15–31) (1975)
51. K. Zierold, X-ray microanalysis of freeze-dried and frozen-hydrated cryosections. J Electron
Microsc. Tech. 9(1), 65–82 (1988). doi:10.1002/jemt.1060090107
52. R.D. Leapman, Detecting single atoms of calcium and iron in biological structures by
electron energy-loss spectrum-imaging. J. Microsc. 210(1), 5–15 (2003). doi:10.1046/j.
1365-2818.2003.01173.x
53. M.T. Otten, D.J. Stenzel, D.R. Cousens, B.M. Humbel, J.L.M. Leunissen, Y.D. Stierhof, W.
M. Busing, High-angle annular dark-field STEM imaging of immunogold labels. Scanning
14(5), 282–289 (1992). doi:10.1002/sca.4950140507
54. U. Ziese, C. Kubel, A. Verkleij, A.J. Koster, Three-dimensional localization of ultrasmall
immuno-gold labels by HAADF-STEM tomography. J. Struct. Biol. 138(1–2), 58–62
(2002). doi:10.1016/S1047-8477(02)00018-7
55. A.A. Sousa, M.A. Aronova, Y.C. Kim, L.M. Dorward, G. Zhang, R.D. Leapman, On the
feasibility of visualizing ultrasmall gold labels in biological specimens by STEM
tomography. J. Struct. Biol. 159(3), 507–522 (2007). doi:10.1016/j.jsb.2007.06.006
56. P. Gentsch, H. Gilde, L. Reimer, Measurement of the top bottom effect in scanning
transmission electron microscopy of thick amorphous specimens. J. Microsc. 100(1), 81–92
(1974). doi:10.1111/j.1365-2818.1974.tb03915.x
57. D.J. Smith, J.M. Cowley, Aperture contrast in thick amorphous specimens using scanning
transmission electron microscopy. Ultramicroscopy 1(2), 127–136 (1975). doi:10.1111/epi.
12023
2 STEM Tomography in Biology
57