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60. A.E. Yakushevska, M.N. Lebbink, W.J.C. Geerts, L. Spek, E.G. van Donselaar, K.A.
Jansen, B.M. Humbel, J.A. Post, A.J. Verkleij, A.J. Koster, STEM tomography in cell
biology. J. Struct. Biol. 159(3), 381–391 (2007). doi:http://dx.doi.org/10.1016/j.jsb.2007.04.
006
61. F. Zemlin, Dynamic focussing for recording images from tilted samples in small-spot
scanning with a transmission electron microscope. J. Electron Microsc. Tech. 11(4), 251–
257 (1989). doi:10.1002/jemt.1060110404
62. K.H. Downing, Automatic focus correction for spot-scan imaging of tilted specimens.
Ultramicroscopy 46(1–4), 199–206 (1992)
63. D.N. Mastronarde, Automated electron microscope tomography using robust prediction of
specimen movements. J. Struct. Biol. 152(1), 36–51 (2005). doi:10.1016/j.jsb.2005.07.007
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Recent advances in electron tomography: TEM and HAADF-STEM tomography for
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(2005). doi:10.1017/S1431927605050361
65. J. Feng, A.P. Somlyo, A.V. Somlyo, Z. Shao, Automated electron tomography with
scanning transmission electron microscopy. J. Microsc. 228(Pt 3), 406–412 (2007). doi:10.
1111/j.1365-2818.2007.01859.x
66. L. Bonetta, Zooming in on electron tomography. Nat. Meth. 2(2), 139–145 (2005). doi:10.
1038/nmeth0205-139
67. P.A. Midgley, M. Weyland, 3D electron microscopy in the physical sciences: The
development of Z-contrast and EFTEM tomography. Ultramicroscopy 96(3–4), 413–431
(2003). doi:10.1016/S0304-3991(03)00105-0
68. J. Loos, E. Sourty, K. Lu, B. Freitag, D. Tang, D. Wall, Electron tomography on
micrometer-thick specimens with nanometer resolution. Nano. Lett. 9(4), 1704–1708 (2009).
doi:10.1021/nl900395g
69. J. Biskupek, J. Leschner, P. Walther, U. Kaiser, Optimization of STEM tomography
acquisition-a comparison of convergent beam and parallel beam STEM tomography.
Ultramicroscopy 110(9), 1231–1237 (2010). doi:10.1016/j.ultramic.2010.05.008
70. A.A. Sousa, A.A. Azari, G.F. Zhang, R.D. Leapman, Dual-axis electron electron
tomography of biological specimens: extending the limits of specimen thickness with
bright-field STEM imaging. J. Struct. Biol. 174(1), 107–114 (2011). doi:10.1016/j.jsb.2010.
10.017
71. S. Boonrungsiman, E. Gentleman, R. Carzaniga, N.D. Evans, D.W. McComb, A.E. Porter,
M.M. Stevens, The role of intracellular calcium phosphate in osteoblast-mediated bone
apatite formation. Proc. Natl. Acad. Sci. U S A 109(35), 14170–14175 (2012). doi:10.1073/
pnas.1208916109
72. G.M. Khalifa, D. Kirchenbuechler, N. Koifman, O. Kleinerman, Y. Talmon, M. Elbaum, L.
Addadi, S. Weiner, J. Erez, Biomineralization pathways in a foraminifer revealed using a
novel correlative cryo-fluorescence–SEM–EDS technique. J. Struct. Biol. doi:http://dx.doi.
org/10.1016/j.jsb.2016.01.015
73. R.D. Leapman, S.Q. Sun, Cryoelectron energy-loss spectroscopy—observations on vitrified
hydrated specimens and radiation damage. Ultramicroscopy 59(1–4), 71–79 (1995). doi:10.
1016/0304-3991(95)00019-w
74. L.R. Comolli, K.H. Downing, Dose tolerance at helium and nitrogen temperatures for whole
cell electron tomography. J. Struct. Biol. 152(3), 149–156 (2005). doi:10.1016/j.jsb.2005.08.
004
58
S. G. Wolf et al.